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Conventional and dual competitive binding analyses of thyroxine using the Thyopac-4 kit
  1. Satoshi Hamada,
  2. Kanji Torizuka,
  3. Tetsuo Uete
  1. Radioisotope Research Centre, Kyoto University, Kyoto, Japan
  2. Department of Radiology, Kyoto University School of Medicine, Kyoto, Japan
  3. Division of Clinical Biochemistry, Kitano Hospital, Osaka, Japan


    A simple method is described for the simultaneous determinations of thyroxine and the effective thyroxine ratio (ETR) using a single kit of Thyopac-4. Thyopac ETR values obtained were 1·00 ± 0·03 for normal subjects; 1·22 ± 0·11 for hyperthyroid; 0·87 ± 0·04 for hypothyroid; and 1·02 ± 0·05 for pregnant women. Cases with TBG deficiency and the nephrotic syndrome indicated normal values. The value (x) was correlated significantly (γ = + 0·961) with the free Thyopac index (y), representing a regression equation of y = 71·7x2 − 88·4x + 24·2. Thyopac ETR was shown to be useful in differentiating normal thyroid states from abnormal, irrespective of changes in T4-binding globulin (TBG). Further, the T4 value obtained simultaneously made it possible to detect changes in the TBG level by noting an inconsistent value for the effective thyroxine ratio.

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    • This paper was presented in part at the annual meeting of the Japan Endocrinological Society, April 9-11, 1973.