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Evaluation and utilization of a kinetic enzyme direct measuring photometer.
  1. P L Wolf


    We have recently utilized a prototype model of the Beckman Enzyme Activity Analyzer System-TR in our laboratory measuring various serum enzyme activities which include: alkaline phosphatase (ALP), E.C.; creatine kinase (CK), E.C.; hydroxybutyrate dehydrogenase (HBD), E.C.; lactate dehydrogenase (LD), E.C.; aspartate transaminase (AST), E.C.; and alanine transaminase (ALT), E.C. Precision was found to be good. Sample activities could be measured as high as 1000 IU/1. The carryover studies fell within 2 SD of the means of the enzyme control studies. Coefficients of variation for ALP and CK were in the ranges of 0-40-2-14% and 0-52-4-30%, respectively. Correlation studies were done with GemSAEC and Gilford 300 N Spectrophotometer and the results were accurate, precise, and reproducible.

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