Article Text

Download PDFPDF
Centrifugation techniques and reagent strips in the assessment of microhaematuria.
  1. S C Freni,
  2. G J Heederik,
  3. C Hol


    During a survey for bladder cancer in a healthy male population a surprisingly high incidence of significant microhaematuria was found and no urinary sample was completely free of blood. A study of the sensitivity of the different techniques for assessing microhaematuria seemed necessary. A semiquantitative centrifugation technique, developed for cytological purposes, was found to be the most sensitive. Blood could be detected in concentrations of less than 50 RBC/ml urine. With conventional analysis, performed carefully, the minimum detectable concentration was about 500 RBC/ml. Reagent strips, based on the orthotholidin peroxidase reaction and developed specifically for urine analysis, gave positive reactions in minimum concentrations of 1 000 000 RBC/ml (original Hemastix), 50 000 RBC/ml (new Hemastix), and 20 000 RBC/ml (Sangur-Test). Positive scores with lower concentrations could be obtained with the Sangur-Test strip when it was read with a magnifying glass. However, the degree of erythrocyturia that may be regarded as physiological and that which is pathological has yet to be defined.

    Statistics from

    Request Permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.