The difficulties associated with the isolation of pure C1q in sufficient amounts are reflected by the substantial number of isolation procedures, which are being published. The two major problems are a low yield and contaminating immunoglobulins. In addition, some isolation protocols appear to produce C1q contaminated with an inhibitor (C1q-INH). The present isolation protocol involves precipitation of C1q by DNA, chromatography using Sephadex QAE A 50 followed by Con A affinity chromatography. By this combination of purification steps maximal advantage was taken of the cationic properties and high carbohydrate content of the C1q molecule. The yield was 1-2 mg C1q per 100 ml serum. The isolated C1q was free of any demonstrable contaminants as demonstrated by Ouchterlony double diffusion and polyacrylamide gel electrophoresis.
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