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A modified tetrazolium reaction for identifying malignant cells from gastric and colonic cancer
  1. KS Ibrahim,
  2. OAN Husain,
  3. L Bitensky*,
  4. J Cha Yen*
  1. Cytology Department, Charing Cross Hospital, London W6 8RF
  2. * Division of Cellular Biology, Kennedy Institute of Rheumatology, London W6 7DW


    When non-malignant cells were reacted for glucose-6-phosphate dehydrogenase activity, with neotetrazolium chloride as the indicator of the activity, oxygen competed with the neotetrazolium and nullified the reaction. In contrast, about 30% of the activity was retained in malignant cells, in sections and in smears, from cancer of the stomach or colon. This could provide the basis of a qualitative (black-or-white) functional test for distinguishing malignant cells in these conditions.

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