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Gas chromatographic identification of Clostridium difficile and detection of cytotoxin from a modified selective medium.
  1. P N Levett,
  2. K D Phillips


    A modification of an existing selective medium for Clostridium difficile is described. Inclusion in the medium of DL nor-leucine and p-hydroxyphenylacetic acid enables identification of C difficile to be made directly from primary isolation plates by gas chromatographic detection of caproic acid and p-cresol. Plugs of agar withdrawn from the selective medium also allow the detection of cytotoxin production in vitro.

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