Article Text

Download PDFPDF

Sensitive system for visualising biotinylated DNA probes hybridised in situ: rapid sex determination of intact cells.
  1. J Burns,
  2. V T Chan,
  3. J A Jonasson,
  4. K A Fleming,
  5. S Taylor,
  6. J O McGee


    A fast and sensitive method for detecting biotinylated deoxyribonucleic acid (DNA) probes was used for sex determination of cells and tissues by in situ hybridisation of a probe "specific" for the Y chromosome (pHY 2.1). Within 24 hours this procedure visualizes the Y chromosome in fetal and adult cells and tissue, without background noise. This procedure should facilitate antenatal determination of sex on small numbers of uncultured cells. The sensitivity of the procedure also permits the chromosomal assignment of genes present in low copy number.

    Statistics from

    Request Permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.