Article Text

Download PDFPDF

Sensitive in situ hybridisation technique using biotin-streptavidin-polyalkaline phosphatase complex.
  1. F A Lewis,
  2. S Griffiths,
  3. R Dunnicliff,
  4. M Wells,
  5. N Dudding,
  6. C C Bird


    A sensitive in situ hybridisation technique, using a biotin-streptavidin-polyalkaline phosphatase complex detection system, was successfully applied to smears of fresh cultured cells, frozen sections, and formalin fixed paraffin processed tissue: the procedure was successful for DNA-DNA hybridizations using a variety of DNA probes. The detection method is rapid, reliable, and economical producing a purplish-blue precipitate at the site of hybridisation and clearly visible by low power light microscopy.

    Statistics from

    Request Permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.