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Improved silver staining of nucleolar organiser regions in paraffin wax sections using an inverted incubation technique.
  1. G Coghill,
  2. A Grant,
  3. J M Orrell,
  4. J Jankowski,
  5. A T Evans
  1. Department of Pathology, Ninewells Hospital and Medical School, Dundee.


    A new simple modification to the silver staining of nucleolar organiser regions (AgNORs) was devised which, by performing the incubation with the slide inverted, results in minimal undesirable background staining, a persistent problem. Inverted incubation is facilitated by the use of a commercially available plastic coverplate. This technique has several additional advantages over other published staining protocols. In particular, the method is straightforward, fast, and maintains a high degree of contrast between the background and the AgNORs.

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