Article Text

Download PDFPDF
Latex agglutination for rapid detection of Pseudomonas pseudomallei antigen in urine of patients with melioidosis.
  1. M D Smith,
  2. V Wuthiekanun,
  3. A L Walsh,
  4. N Teerawattanasook,
  5. V Desakorn,
  6. Y Suputtamongkol,
  7. T L Pitt,
  8. N J White
  1. Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.


    A latex agglutination test for the detection of Pseudomonas pseudomallei antigen in urine was evaluated for the rapid diagnosis of melioidosis. With unconcentrated urine, antigen was detected in only 18% of patients with melioidosis overall. However, when urine was concentrated 100-fold, antigen was detected in 47% overall and in 67% of patients with septicaemia or disseminated infection, in whom a rapid diagnosis is most important. The specificity of the test was 100%. These results compared favourably with an enzyme immunoassay. This latex agglutination test is a simple, rapid and highly specific method of diagnosing melioidosis, and will be particularly useful in areas with limited laboratory facilities.

    Statistics from

    Request Permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.