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The absence of a B allele in acquired B blood group phenotype confirmed by a DNA based genotyping method.
  1. S P Yip,
  2. W L Choy,
  3. C W Chan,
  4. C H Choi
  1. Department of Health Science, Hong Kong Polytechnic University, Kowloon.


    A case of an 87 year old woman with carcinomatous sigmoid colon, found to be of group A1 with acquired B status, is reported. The acquired B phenotype was confirmed by the absence of a B allele in the patient using denaturing gradient gel electrophoresis of a DNA fragment amplified from the ABO locus by the polymerase chain reaction.

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