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Abstract
Aims: To describe the laboratory confirmation of meningococcal disease, using culture and non-culture based techniques, between 1993 and 1999 as part of a national service in Scotland.
Methods: Samples from patients with suspected meningococcal disease in Scotland were analysed by culture and non-culture based techniques to gain a laboratory confirmation of disease. Data were analysed to establish the number of disease cases, the serogroups of the organisms involved, and the importance of the techniques used.
Results: Between 1993 and 1999, there was a total of 1749 notified cases of meningococcal disease in Scotland. Culture based methods provided a laboratory confirmation of 788 cases whereas non-culture techniques confirmed 461 cases.
Conclusions: Non-culture techniques were a useful addition to culture based techniques in Scotland and improved the dataset required for public health management, disease surveillance, and vaccine policy.
- meningococcal disease
- Neisseria meningitidis
- non-culture diagnosis
- CSF, cerebrospinal fluid
- MenC, serogroup C meningococcal conjugate
- OMP, outer membrane protein
- PBS, phosphate buffered saline
- PCR, polymerase chain reaction
- SCIEH, Scottish Centre for Infection and Environmental Health
- SMPRL, Scottish Meningococcus and Pneumococcus Reference Laboratory