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Misidentification of a genomovar of Burkholderia cepacia by recA restriction fragment length polymorphism
  1. J E Moore1,
  2. B C Millar1,
  3. J Xu1,
  4. M Crowe1,
  5. A O B Redmond2,
  6. J S Elborn3
  1. 1Northern Ireland Public Health Laboratory, Department of Bacteriology, Belfast City Hospital, Belfast BT9 7AD, Northern Ireland, UK
  2. 2Northern Ireland Regional Paediatric Cystic Fibrosis Centre, Royal Belfast for Sick Children, Belfast, Northern Ireland, UK
  3. 3Northern Ireland Regional Adult Cystic Fibrosis Centre, Belfast City Hospital
  1. Correspondence to:
 Dr J E Moore, Northern Ireland Public Health Laboratory, Department of Bacteriology, Belfast City Hospital, Belfast BT9 7AD, Northern Ireland, UK;
 jemoore{at}niphl.dnet.co.uk

Abstract

An 8 year old girl with cystic fibrosis presented with a pulmonary exacerbation from which Burkholderia cepacia was cultured. Subsequent polymerase chain reaction restriction fragment length polymorphism analysis of the recA gene suggested the presence of B cepacia Genomovar V (Burkholderia vietnamiensis); however, on subsequent sequence typing, this isolate was confirmed as B cepacia Genomovar IIIb. This report outlines the potential difficulties in the correct characterisation of the various genomovars within the B cepacia complex of organisms, which has particularly important implications for patient segregation and infection control.

  • Burkholderia cepacia
  • cystic fibrosis, Genomovar, infection control, recA
  • CF, cystic fibrosis
  • PCR, polymerase chain reaction
  • RFLP, restriction fragment length polymorphism

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