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Reliability of the tissue microarray based FISH for evaluation of the HER-2 oncogene in breast carcinoma
  1. D Gancberg1,
  2. A Di Leo1,
  3. G Rouas1,
  4. T Järvinen3,
  5. A Verhest2,
  6. J Isola3,
  7. M J Piccart1,
  8. D Larsimont2
  1. 1Translational Research Unit of the Chemotherapy Department, Jules Bordet Institute, 1000 Brussels, Belgium
  2. 2Pathology Department, Jules Bordet Institute
  3. 3Laboratory of Cancer Genetics, Institute of Medical Technology, Tampere University Hospital and University of Tampere, Tampere, Finland
  1. Correspondence to:
 Dr D Gancberg, Translational Research Unit, c/o Department of Chemotherapy, 1 Rue Heger-Bordet, 1000 Brussels, Belgium;


Aims: Tumour tissue microarray allows the analysis of hundreds of tumour samples simultaneously on a single microscope slide. However, the extremely small tissue samples taken from the original tissue may not always be representative of the entire tumour.

Methods: The reliability of this new technology was investigated by analysing HER-2 oncogene amplification by fluorescence in situ hybridisation (FISH) from representative slides of the whole tumour and small tissue core biopsies from 29 invasive breast tumours.

Results: The tissue microarray method had high accuracy; in only one of 29 cases (3.4%; 95% confidence interval, 0% to 10%) were the results discordant with whole tumour analysis.

Conclusion: Tumour microarray is a highly reliable method for analysing HER-2 oncogene amplification by FISH in human breast tumours.

  • fluorescence in situ hybridisation
  • tissue microarray
  • breast carcinoma
  • HER-2
  • FISH, fluorescence in situ hybridisation
  • IHC, immunohistochemistry
  • SSC, saline sodium citrate

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