Article Text

Download PDFPDF

The expression of bone morphogenetic proteins in osteosarcoma and its relevance as a prognostic parameter
Free
  1. I Sulzbacher1,
  2. P Birner1,
  3. K Trieb2,
  4. E Pichlbauer1,
  5. S Lang1
  1. 1Clinical Institute of Pathology, University of Vienna, Medical School, Waehringer Guertel 18–20, A-1090 Vienna, Austria
  2. 2University Clinic of Orthopedics, University of Vienna
  1. Correspondence to:
 Dr I Sulzbacher, Clinical Institute of Pathology, University of Vienna Medical School, Waehringer Guertel 18–20, A-1090 Vienna, Austria;
 irene.sulzbacher{at}akh-wien.ac.at

Abstract

Aims: The expression of bone morphogenetic proteins (BMPs) was analysed in 47 osteosarcomas to determine differences in the expression of BMP subtypes and to correlate expression with response to chemotherapy, in addition to the disease free and overall survival of patients.

Methods: The expression of BMPs was examined immunohistochemically in 47 biopsy specimens of osteosarcoma using commercially available antibodies against different subtypes (BMP-2/4 (A-20), BMP-3 (N-19), BMP-4 (N-19), BMP-5 (N-19), BMP-6 (N-19), BMP-7 (N-19), BMP-8 (N-19)). The avidin–biotin–immunoperoxidase method was used for all antibodies.

Results: The expression of BMP subtypes varied considerably: 28 of the 47 tumours expressed BMP-2/4, 24 expressed BMP-3, 41 expressed BMP-5, 31 expressed BMP-6, 43 expressed BMP-7, and 42 expressed BMP-8. High expression of BMP-6 was found in those parts of the osteosarcoma with a chondroid differentiation (p = 0.016, Mann-Whitney test). No correlation was observed between the response to chemotherapy and the expression of BMPs (p > 0.05, Mann-Whitney test). Univariate analysis showed no correlation between overall survival or progression free survival and the expression of BMPs (p > 0.05, log rank test).

Conclusions: BMP-7 and BMP-8 are highly expressed in osteosarcoma. Moreover, high expression of BMP-6 correlates with a chondroid differentiation. In contrast to conclusions derived from previous studies in which small numbers of tumours were investigated, these results indicate that the expression of BMPs does not help to predict the outcome of patients.

  • bone morphogenetic protein
  • osteosarcoma
  • prognosis
  • immunohistochemistry
  • BMP, bone morphogenetic protein
  • COSS, cooperative osteosarcoma study
  • TGF-β, transforming growth factor β
View Full Text

Statistics from Altmetric.com

Footnotes

    Request Permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.