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Heparin and chondroitin sulfate are both proteoglycans. Therefore, we carried out this study to examine whether heparin-like granular deposits, which frequently impair the morphological assessment of bone marrow touch preparations, could result from the inclusion of cartilage in the bone marrow biopsy.
Touch imprints were made from bone marrow biopsies taken from the posterior superior iliac spine using Jamshidi needles.1,2 Bone marrow touch preparations were stained with Jenner Giemsa, and decalcified bone marrow biopsies were stained with haematoxylin and eosin.
Two hundred and twenty consecutive bone marrow touch preparations and the corresponding biopsies were evaluated for the presence of purple granular deposits and cartilage, respectively. Inspection of trephine biopsies for cartilage was done without knowledge of the touch imprint findings.
The χ2 test was carried out to test whether granular deposits and/or bone marrow biopsy cartilage were more common in children than in adults.
One hundred and ten of the 220 touch preparations …
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