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A simple method for the construction of small format tissue arrays
  1. A Hidalgo1,
  2. P Piña1,
  3. G Guerrero2,
  4. M Lazos3,
  5. M Salcedo1
  1. 1Laboratorio de Oncología Genómica, Unidad de Investigación Médica en Enfermedades Oncológicas, Centro Médico Nacional Siglo XXI-IMSS, México DF, 06720
  2. 2Departamento de Anatomía Patológica, Hospital de la Mujer, S.S., Mexico 11340
  3. 3Departamento de Patología, Hospital General de México, S.S., Mexico 06726
  1. Correspondence to:
 Dr M Salcedo, Laboratorio de Oncología Genómica, Unidad de Investigación Médica en Enfermedades Oncológicas, Coordinación de Investigación en Salud, Hospital de Oncología, Centro Médico Siglo XXI-IMSS, Av. Cuauhtémoc 330, Col. Doctores, México DF, 06720;
 alfhm{at}; maosal89{at}


Tissue arrays can evaluate molecular targets in high numbers of samples in parallel. Array construction presents technical difficulties and tissue arrayers are expensive, particularly for small and medium sized laboratories. This report describes a method for the construction of 36 sample arrays using widely available materials. A blunted 16 gauge needle for bone marrow aspiration was used to extract paraffin wax cylinders and manually define a 6 × 6 matrix on a blank paraffin wax block. Tissue cores from 36 paraffin wax embedded premalignant lesions and invasive cervical carcinomas were injected into the matrix using a 14 gauge needle. This tissue array was sectioned using a standard microtome and used for the immunodetection of CD44 variant 9 and interleukin 18 with satisfactory results. This method can be applied in any laboratory, without the need of specialised equipment, offering a good alternative for the wider application of tissue arrays.

  • tissue arrays
  • marker validation
  • CD44V9, CD44 variant 9
  • G, gauge
  • IL, interleukin

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