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A comparison of IgG anti-rubella activity in frozen serum stored in primary gel separation tubes or secondary tubes
  1. V Ellis1,
  2. A Charlett2,
  3. R Bendall1
  1. 1Department of Clinical Microbiology, Royal Cornwall Hospital, Penventinnie Lane, Treliske, Truro, Cornwall TR1 3LQ, UK
  2. 2Statistics Unit, Health Protection Agency, Communicable Disease Surveillance Centre, 61 Colindale Avenue, London NW9 5EQ, UK
  1. Correspondence to:
 Dr R Bendall
 Department of Clinical Microbiology, Royal Cornwall Hospital, Penventinnie Lane, Treliske, Truro, Cornwall TR1 3LQ, UK; richard.bendallcornwall.nhs.uk

Abstract

Aims: To determine the suitability of primary gel separation tubes for the storage of frozen sera intended for serological testing.

Methods: Blood samples from 102 patients were collected into gel separation tubes. The sera from these samples were split between the primary gel separation tubes and conventional plastic storage tubes and frozen. A year later, the tubes were thawed and anti-rubella IgG concentrations were compared for the serum pairs using the Wilcoxon signed rank test.

Results: No significant difference was detected between the two storage methods.

Conclusions: Frozen storage of serum samples in primary gel separation tubes is a practical alternative to storing separated sera in secondary containers. Adopting this practice has advantages for laboratories in reducing specimen handling and reducing errors in labelling stored samples.

  • Serum
  • gel separation
  • frozen storage
  • antibodies

https://doi.org/10.1136/jcp.57.1.104

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