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Characterisation of hexon and fibre genes of a novel strain of adenovirus involved in epidemic keratoconjunctivitis
  1. A K Adhikary1,
  2. T Inada1,
  3. J Numaga2,
  4. E Suzuki3,
  5. H Ushijima3,
  6. U Banik1,
  7. A Mukouyama1,
  8. S Matsuno1,
  9. N Okabe1
  1. 1Infectious Disease Surveillance Centre, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan
  2. 2Department of Ophthalmology, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
  3. 3Department of Developmental Medical Sciences, Graduate School of Medicine, The University of Tokyo
  1. Correspondence to:
 Dr A K Adhikary
 Mailing, Infectious Disease Surveillance Centre, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan; arunnih.go.jp

Abstract

Aims: To characterise a novel strain (M86) of adenovirus (Ad) involved in epidemic keratoconjunctivitis (EKC).

Methods/Results: The virus strain was neutralised by antisera to both Ad35 and Ad11. Restriction endonuclease analysis of genomic DNA showed 98% and 88% homology with Ad11 and Ad35, respectively. The deduced amino acid sequence of the hypervariable regions of (HVRs) of the hexon gene showed a higher homology with Ad35 (94.4%) than with Ad11 (83.7%). However, it was 100% homologous to Ad35 in HVRs 1, 2, 3, and 6 and to Ad11 in HVRs 4 and 6. In the fibre knob, the isolate was more homologous to Ad11 (99.4%) than to Ad35 (29.1%).

Conclusion: This novel strain of adenovirus showed similarities with both Ad11 and Ad35. The isolation of a novel strain like Ad35+11 is important because of its association with EKC.

  • adenovirus
  • restriction endonuclease analysis
  • sequence analysis
  • AA, amino acid
  • Ad, adenovirus
  • EKC, epidemic keratoconjunctivitis
  • HVR, hypervariable region

https://doi.org/10.1136/jcp.57.1.95

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