Aims: To assess different laboratory methods for the identification of Entamoeba histolytica in clinical samples.
Methods: Antigen detection enzyme linked immunosorbent assay, polymerase chain reaction solution hybridisation enzyme linked immunoassay (PCR-SHELA), and a commercial Lightcycler PCR were compared using 101 stool and pus samples.
Results: Fifteen of the 101 samples were positive for E histolytica by one or more method. There were discrepancies between the results in five of these 15 samples when the assays were compared.
Conclusions: All three methods performed adequately, so that the choice of assay will depend on each individual laboratory’s budget and projected turnaround time.
- ELISA, enzyme linked immunosorbent assay
- LC, Lightcycler
- PCR, polymerase chain reaction
- SHELA, solution hybridisation enzyme linked immunoassay
- Entamoeba dispar
- Entamoeba histolytica
- antigen detection
- polymerase chain reaction
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