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An immunohistochemical analysis to evaluate an inverse correlation between Runx2/Cbfa1 and NFκB in human osteosarcoma
  1. V B Andela1,
  2. F Siddiqui2,
  3. A Groman3,
  4. R N Rosier2
  1. 1The James P Wilmot Cancer Center, University of Rochester Medical Center, 601 Elmwood Avenue Box 665, Rochester, NY, 14642, USA
  2. 2The Department of Orthopaedics, Center for Musculoskeletal Research, University of Rochester Medical Center
  3. 3The Department of Biostatistics, University of Rochester Medical Center
  1. Correspondence to:
 Dr V B Andela
 University of Rochester Medical Center, 601 Elmwood Avenue Box 665, Rochester, NY, 14642, USA; v_andelayahoo.com

Abstract

Background: Dominant negative inhibition of nuclear factor κB (NFκB) signalling activity in a human osteosarcoma cell line (Saos2) results in malignant reversion and the induction of the osteoblast differentiating transcription factor, Runx2/Cbfa1. This observation suggests that there is an inverse relation between a transcription factor associated with malignant progression and chemoresistance (NFκB) and an osteoblast differentiating transcription factor (Runx2/Cbfa1).

Aims: To assess and correlate Runx2/Cbfa1 and NFκB (p65) immunoreactivity in human osteosarcoma.

Methods: Runx2/Cbfa1 and NFκB (p65) immunoreactivity was assessed on 11 paraffin wax embedded archival specimens of human primary osteosarcoma by standard immunohistochemical methods and scored on a scale of 0–3. A Pearson correlation analysis between Runx2/Cbfa1 and NFκB (p65) scores was established.

Results: Runx2/Cbfa1 was expressed constitutively in all pathology specimens of human osteosarcoma. Of note, a chondroblastic osteosarcoma showed the highest Runx2/Cbfa1 immunoreactivity. A Pearson correlation did not support an inverse correlation between Runx2/Cbfa1 and NFκB (p65) scores (r  =  0.57) in human osteosarcoma.

Conclusion: Runx2/Cbfa1 immunoreactivity does not inversely correlate with NFκB immunoreactivity, and thus cannot serve as an indirect measure of NFκB activity or an independent predictive or prognostic indicator.

  • NFκB, nuclear factor κB
  • Rb, retinoblastoma protein
  • differentiation
  • nuclear factor κB
  • osteosarcoma
  • Runx2/Cbfa1
  • transformation

https://doi.org/10.1136/jcp.2004.017640

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