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An immunohistochemical analysis to evaluate an inverse correlation between Runx2/Cbfa1 and NFκB in human osteosarcoma

Abstract

Background: Dominant negative inhibition of nuclear factor κB (NFκB) signalling activity in a human osteosarcoma cell line (Saos2) results in malignant reversion and the induction of the osteoblast differentiating transcription factor, Runx2/Cbfa1. This observation suggests that there is an inverse relation between a transcription factor associated with malignant progression and chemoresistance (NFκB) and an osteoblast differentiating transcription factor (Runx2/Cbfa1).

Aims: To assess and correlate Runx2/Cbfa1 and NFκB (p65) immunoreactivity in human osteosarcoma.

Methods: Runx2/Cbfa1 and NFκB (p65) immunoreactivity was assessed on 11 paraffin wax embedded archival specimens of human primary osteosarcoma by standard immunohistochemical methods and scored on a scale of 0–3. A Pearson correlation analysis between Runx2/Cbfa1 and NFκB (p65) scores was established.

Results: Runx2/Cbfa1 was expressed constitutively in all pathology specimens of human osteosarcoma. Of note, a chondroblastic osteosarcoma showed the highest Runx2/Cbfa1 immunoreactivity. A Pearson correlation did not support an inverse correlation between Runx2/Cbfa1 and NFκB (p65) scores (r  =  0.57) in human osteosarcoma.

Conclusion: Runx2/Cbfa1 immunoreactivity does not inversely correlate with NFκB immunoreactivity, and thus cannot serve as an indirect measure of NFκB activity or an independent predictive or prognostic indicator.

  • NFκB, nuclear factor κB
  • Rb, retinoblastoma protein
  • differentiation
  • nuclear factor κB
  • osteosarcoma
  • Runx2/Cbfa1
  • transformation

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