Identification of the causative organism in invasive fungal infections is difficult. Accurate and reliable diagnostic methods are required in light of the increasing incidence of emerging fungal infections.¹ We recently described a method for speciating fungi in formalin fixed, paraffin wax embedded tissue sections.² DNA is extracted using a modification of the protocol for the TaKaRa DEXPAT™ kit (TaKaRa Biomedicals, Cambrex Biosciences, Wokingham, UK), with additional steps using lyticase and ethanol precipitation.² Amplification of the DNA is performed using panfungal primers and a method based on that described by Einsele et al.³ Identification to species level can be achieved by Southern hybridisation with a probe that binds Aspergillus fumigatus, …