Article Text

Download PDFPDF
Analysis of Bcl-2 protein expression in choroidal melanomas
  1. M R Hussein
  1. Correspondence to:
 Dr M R Hussein
 Pathology Department, School of Medicine, Assuit University, Assuit, Egypt;


Background: Bcl-2 protooncogene alterations are involved in tumorigenesis and may have prognostic ramifications.

Aims: To investigate normal ocular structures and choroidal melanoma for: (1) Bcl-2 protein expression (semiquantitative staining values: SI, staining intensity; PP, percentage of positive cells; and IRS, immunoreactivity score) and (2) any associations between the staining values and clinicopathological features in these lesions.

Materials/Methods: Bcl-2 protein expression was analysed in 24 choroidal melanomas using immunoperoxidase staining methods.

Results: Bcl-2 protein expression was seen in corneal epithelium, lens epithelium, the ciliary body, and retinal cells. In these structures, the mean (SEM) values were: 1.1 (0.1), 1.6 (0.3), 1.1 (0.1), and 2.3 (0.3), respectively, for SI; 1.6 (0.2), 1.7 (0.1), 1.7 (0.2), and 1.7 (0.2) for PP, respectively; and 1.9 (0.4), 2.7 (0.5), 1.9 (0.1), and 4.0 (0.8), respectively, for IRS. Based on Bcl-2 immunoreactivity, the lesions were divided into two groups. The first group comprised 12 tumours with Bcl-2 expression. Bcl-2 expression was significantly higher in this group compared with normal ocular structures (1.5 (0.1) v 2.8 (0.2), 1.7 (0.1) v 3.5 (0.1), and 2.6 (0.3) v 9.3 (0.9) for mean (SEM) SI, PP, and IRS scores, respectively; p  =  0.00). The second group comprised 12 tumours lacking Bcl-2 protein expression. There was no significant correlation between Bcl-2 protein expression and most of the clinicopathological features of these lesions.

Conclusions: Bcl-2 protein expression is altered in choroidal melanomas.

  • IRS, immunoreactivity score
  • PP, percentage of positive cells
  • SI, staining intensity
  • choroidal melanoma
  • Bcl-2
  • immunoperoxidase

Statistics from

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.


  • Former address: University of Wisconsin Medical School and William S. Middleton Memorial Veteran Hospital, Madison, WI 53705, USA