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  • Coexpression of an unusual form of the EWS–WT1 fusion transcript and interleukin 2/15 receptor βmRNA in a desmoplastic small round cell tumour

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Coexpression of an unusual form of the EWS–WT1 fusion transcript and interleukin 2/15 receptor βmRNA in a desmoplastic small round cell tumour
  1. Y Nakanishi1,
  2. T Oinuma1,
  3. M Sano1,
  4. F Fuchinoue1,
  5. K Komatsu2,
  6. T Seki2,
  7. Y Obana1,
  8. M Tabata1,
  9. K Kikuchi1,
  10. M Shimamura3,
  11. K Ohmori3,
  12. N Nemoto1
  1. 1Department of Pathology, Nihon University School of Medicine, Tokyo, Japan
  2. 2Pathology Laboratory, Nihon University Itabashi Hospital, Tokyo
  3. 3Department of Thoracic Surgery, Nihon University School of Medicine
  1. Correspondence to:
 Dr N Nemoto
 Pathology Laboratory, Nihon University Itabashi Hospital, 30-1 Oyaguchi-kamimachi Itabasi-ku, Tokyo 173-8610, Japan; nemo{at}med.nihon-u.ac.jp

Abstract

Background: The β chain of the interleukin 2/15 receptor (IL-2/15Rβ) is induced by the expression of the EWS–WT1. A case of desmoplastic small round cell tumour (DSRCT) expressing only an unusual EWS-WT1 treated by us is reported here.

Aim: To characterise an unusual form of EWS–WT1.

Methods: Frozen tissue sections of the axillary tumour were examined using a laser-assisted microdissection technique and reverse transcriptase polymerase chain reaction.

Results: The novel fusion of exon 8 of EWS and the defective exon 10 of WT1 (−KTS) was detected. Although it was an unusual form, the coexpression of the present EWS–WT1, IL-2/15Rβ and Janus kinase (JAK1) mRNA was detected in the tumour cells. IL-2 and signal transducers and activators of transcription (STAT5) mRNA were detected in both tumour and stromal cells.

Conclusion: The induction of the IL-2/15 receptor signalling pathway may contribute to tumorigenesis in DSRCT through a paracrine or an autocrine system, even though the EWS–WT1 was an unusual form.

  • DSRCT, desmoplastic small round cell tumour
  • EWS, Ewing’s sarcoma gene
  • IL-2/15Rβ, interleukin 2/15 receptor β chain
  • JAK, Janus kinase
  • RT-PCR, reverse transcriptase polymerase chain reaction
  • STAT5, signal transducers and activators of transcription
  • WT1, Wilms’s tumour gene

https://doi.org/10.1136/jcp.2005.026245

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    Footnotes

    • Competing interests: None declared.