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Analysis of cerebrospinal fluid for suspected subarachnoid haemorrhage is improved by built-in spectrophotometer software
  1. A Viljoen1,
  2. K S Walker2,
  3. C Ho2,
  4. P J Twomey3
  1. 1Department of Clinical Biochemistry and Immunology, Addenbrookes Hospital, Cambridge, UK
  2. 2Department of Clinical Biochemistry, Royal Infirmary of Edinburgh, Edinburgh, UK
  3. 3Department of Clinical Biochemistry, Ipswich Hospital, Ipswich, UK
  1. Correspondence to:
    A Viljoen
    Department of Clinical Biochemistry and Immunology, Addenbrookes Hospital, Hills Road, Cambridge CB2 2QQ, UK; adie.viljoen{at}

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The spectrophotometric analysis of cerebrospinal fluid has an important role in the diagnosis of subarachnoid haemorrhage.1 The presence of bilirubin, which is the basis for the laboratory identification of in vivo haemorrhage, is assessed by calculating the net bilirubin absorbance (NBA). Guidelines for this practice have recently been published.2 To calculate the NBA, several measurement steps are required: locating the tangential points to construct a baseline; locating the 476-mm position on the scan to construct a perpendicular line; and measuring the distances between the baseline and peak, and correlating this with the distance measurement and absorbance magnitude …

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