Article Text

Download PDFPDF

Skin adnexal neoplasms—part 1: An approach to tumours of the pilosebaceous unit
  1. K O Alsaad,
  2. N A Obaidat,
  3. D Ghazarian
  1. Department of Laboratory Medicine and Pathobiology, University of Toronto and University Health Network, Toronto, Ontario, Canada
  1. Correspondence to:
 Dr D Ghazarian
 Department of Laboratory Medicine and Pathobiology, University of Toronto and University Health Network, Toronto General Hospital, 200 Elizabeth Street, Toronto, Ontario, Canada M5G 2M9; danny.ghazarian{at}


Skin adnexal neoplasms comprise a wide spectrum of benign and malignant tumours that exhibit morphological differentiation towards one or more types of adnexal structures found in normal skin. Most adnexal neoplasms are relatively uncommonly encountered in routine practice, and pathologists can recognise a limited number of frequently encountered tumours. In this review, the first of two, the normal histology of the skin adnexal structures is reviewed, and the histological features of selected but important benign and malignant tumours and tumour-like lesions of pilosebaceous origin discussed, with emphasis on the diagnostic approach and pitfalls in histological diagnosis.

  • BCC, basal cell carcinoma
  • BEPs, basaloid epidermal proliferations
  • BFH, basaloid follicular hamartoma
  • CEA, carcinoembryonic antigen
  • DTE, desmoplastic trichoepithelioma
  • EMA, epithelial membrane antigen
  • GCDEP-15, gross cystic disease fluid protein 15
  • HAS, hidroacanthoma simplex
  • H&E, haematoxylin and eosin
  • HMWK, high molecular weight keratin
  • IHC, immunohistochemical
  • LMWK, low-molecular weight cytokeratin
  • MFT, multiple familial trichoepithelioma
  • NSJ, nevus sebaceous of Jadassohn
  • PAS, periodic acid Schiff
  • PTC, proliferating trichilemmal cyst
  • SAT, Skin adnexal tumours
  • SCAP, syringocystadenoma papilliferum
  • SMA, smooth muscle actin

Statistics from

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.

Skin adnexal tumours (SAT) are a large and diverse group of benign and malignant neoplasms, which exhibit morphological differentiation towards one of the different types of adnexal epithelium present in normal skin: pilosebaceous unit, eccrine and apocrine. SAT may display more than one line of differentiation (hybrid/composite tumours), rendering precise classification of these neoplasms difficult.1 The diagnosis of these mixed SAT relies on histological evaluation, and they are usually classified according to the predominant morphological component. The histogenesis of mixed adnexal tumours is still uncertain; however, the possibility of origin from a pluripotent stem cells is suggestive.2

Most SAT are benign, and local complete surgical excision is curative. However, diagnosing some of these tumours has important implications, as they might be markers for syndromes associated with internal malignancies, such as trichilemmomas in Cowden disease and sebaceous tumours in Muir–Torre syndrome. A malignant counterpart of almost every SAT has been described. These tumours are rare, locally aggressive, and have the potential for nodal involvement and distant metastasis, with a poor clinical outcome. Therefore, establishing a diagnosis of malignancy in SAT is important for therapeutic and prognostic purposes. Because pathologists may not frequently encounter SAT, and owing to their different derivation and broad histogenesis, diagnosing these tumours may be challenging even to an experienced pathologist. In this article, we review the histological features of selected benign, malignant and tumour-like lesions of pilosebaceous origin (box 1), with emphasis on the diagnostic approach and morphological pitfalls in histological evaluation.

Box 1 Skin adnexal neoplasms and neoplastic-like lesions of pilosebaceous origin

Hyperplastic and hamartomatous lesions

  • Hair and hair follicle

    • Basaloid follicular hamartoma

    • Basaloid epidermal proliferation

    • Overlying dermal mesenchymal lesions

    • Trichofolliculoma

    • Sebaceous trichofolliculoma

    • Folliculosebaceous cystic hamartoma

    • Trichodiscoma/fibrofolliculoma

    • Pilar sheath acanthoma

  • Sebaceous glands

    • Sebaceous hyperplasia

    • Nevus sebaceous of Jadassohn

Benign neoplasms

  • Hair and hair follicle

    • Trichofolliculoma

    • Desmoplastic trichoepithelioma

    • Trichoblastoma

    • Trichoblastic fibroma

    • Trichoadenoma

    • Proliferating trichilemmal cyst/pilar tumour

    • Trichilemmoma

    • Desmoplastic trichilemmoma

    • Pilomatricoma/proliferative pilomatricoma

  • Sebaceous glands

    • Sebaceous adenoma

    • Sebaceoma/sebaceous epithelioma

Malignant neoplasms

  • Hair and hair follicle

    • Trichilemmal carcinoma

    • Trichoblastic carcinoma

    • Malignant proliferating trichilemmal cyst

    • Pilomatrix carcinoma

  • Sebaceous glands

    • Sebaceous carcinoma

    • Basal cell carcinoma with sebaceous differentiation


Skin appendages are derived from the ectoderm, and start to develop early during the embryological life. During the fourth week of development, a single-cell-thick ectoderm and underlying mesoderm begin to proliferate, and differentiate towards various structures, including skin appendages. These specialised skin structures are located within the dermis, including the deep dermis, and focally within the subcutaneous fatty tissue. They are represented by three histologically distinct structures: (1) the pilosebaceous unit; (2) the eccrine sweat glands; and (3) the apocrine glands. The distribution and arrangement of these appendages vary from one part of the skin to another, but the overall general basic morphogenesis is maintained. The pilosebaceous units (hair follicle and sebaceous glands) originate from the primary epithelial germs in the epidermis, a collection of deeply basophilic cells in the basal cell layer of the epidermis, protruding into the dermis and surrounded by an aggregate of mesenchymal cells. The hair follicle is a tubular invagination from the epidermis, responsible for the formation of hair, a highly modified keratinised structure. Highly vascular connective tissue papillae, enclosed by bulbous expansion (the hair bulb), are located in the reticular dermis or in the superficial subcutaneous fatty tissue, and form the lower portion of the hair follicle. The inner, mitotically active cells lining the dermal papillae undergo keratinisation to form the hair shaft and internal root sheath. Each hair shaft consists of an innermost medulla, surrounded by a broad, highly keratinised cortical layer, and an outermost thin layer of overlapping keratin, the cuticle. The outer two epidermal cell layers of hair bulb form the external root sheath, which consists of large, glycogen-rich (clear) cells and is separated from the surrounding dermal connective tissue by a thick glassy membrane composed of homogenised fibrous tissue (fig 1).

Figure 1

 Structure of hair bulb: (a) dermal papilla, (b) matrix, (c) melanocytes, (d) medulla, (e) cortex of hair, (f) area of hair cuticle, (g) Huxley’s layer (prominent keratohyalin granules), (h) Henle’s layer (one cell layer), (i) inner root sheath composed of cuticle, Huxley’s and Henle’s layers, (j) outer root sheath and (k) glassy basement membrane.

Developmentally, all sebaceous glands are hair follicle-dependent (except those of the labia minora and glans penis), and originate as a budding of sebaceous glands primordium. The sebaceous glands consist of multiple lobules of rounded cells (sebocytes), filled with lipid-containing vacuoles, and rimmed by a single layer of small, dark germinative cells. The lobules converge on a short duct, which empties the lipid content of degenerated sebocytes into the hair follicle. Sebaceous glands are immunoreactive to low-molecular weight cytokeratin (LMWK), epithelial membrane antigen (EMA) and, to a lesser extent, to the lymphatic marker D2-40. They are negative for S100 protein and carcinoembryonic antigen (CEA).

Eccrine sweat glands originate from epidermal epithelial germs protruding into the dermis similar to those of pilosebaceous units, but contain less mesenchymal condensation. They are distributed almost everywhere in the skin. The sweat-secreting coil glands are tubular and consist of two anatomical portions:

  1. The secretory coil, located in the deep dermis at the junction with the subcutaneous tissue and composed of clear pyramidal cells, and darkly stained cells that are often difficult to identify on light microscopy. These cells express the immunohistochemical stains LMWK, EMA, CEA and S100 protein (basal layer only). A single outer, discontinuous layer of myoepithelial cells resting on a well-defined basement membrane is present, and can be highlighted by immunohistochemical (IHC) analysis for smooth muscle actin (SMA), p63 and calponin.

  2. The excretory part is composed of a straight intradermal portion and an intraepidermal spiral portion (acrosyringium), and consists of a double layer of small cuboidal cells with no underlying myoepithelial layer. The cells in this part may stain with high molecular weight keratin (HMWK) and cytokeratin (CK) 14.

Apocrine glands are seen mainly in the axillae, groin, pubic and perineal regions. In contrast with eccrine glands, apocrine glands develop from an upper bulge in hair follicles that are in the early bulbous peg stage. They have a coiled, tubular excretory portion with widely dilated lumen, lined by cuboidal epithelial cells with eosinophilic cytoplasm and apical snouts (representing decapitation secretion), and an outer discontinuous layer of myoepithelial cells, resting on a prominent basement membrane. The cytoplasm of apocrine glandular cells might contain iron, which can be illustrated using Prussian blue stain. The luminal cells are characteristically immunoreactant to gross cystic disease fluid protein 15 (GCDFP-15). These glands also express androgen receptors, which may be useful in the assessment of lesions suspected to be apocrine carcinoma. The excretory duct of apocrine glands is histologically similar to that of eccrine glands, and drains into the hair shaft. A third type of sweat gland is “apoeccrine” glands. These are atrichial glands which open directly to the skin surface, but their secretory coil is similar to that of apocrine glands.3 These glands are mostly found in the axillary region, and within lesions of the nevus sebaceous of Jadassohn (NSJ)4; they are considered to be the origin of some adnexal lesions, such as syringocystadenoma papilliferum (SCAP),5 and Fox–Fordyce disease.6 The occurrence of mammary-like glands mainly in the anogenital region has been elucidated in recent years and will be discussed further in the second review of cutaneous sweat gland tumours.


In the pathological evaluation of skin specimens for SAT, the pathologist should be provided with sufficient clinical data that may prove to be useful in achieving accurate diagnosis. These include patient’s age and sex, location(s) of the lesion, the rate of tumour growth, whether the lesion is solitary or multiple, and, if present, any associated inherited or systemic diseases. The specimen should be examined grossly and meticulously; the size, growth pattern (nodular or plaque), colour, presence of ulceration, and status of the surgical resection margins should be recorded. The specimen should be thoroughly sampled after painting its resection margins; the lesion should be serially sectioned at 0.3–0.5-cm intervals and submitted in its entirety for histological examination. Sections including tumoral and grossly uninvolved surrounding tissue are relevant to evaluate the growth pattern of the tumour. The deep and peripheral surgical resection margins should be submitted to assure complete excision. It is important to note that a small/superficial biopsy may preclude accurate diagnosis of skin adnexal lesions, and is therefore not advised.

After proper fixation and processing, haematoxylin and eosin (H&E)-stained sections are considered sufficient for routine microscopic evaluation. The presence of cells with coarsely vacuolated cytoplasm and starry nuclei (mulberry cells) within a tumour is indicative of sebaceous differentiation. Follicular differentiation in adnexal tumours is characterised by the presence of proliferation of basaloid bulbar follicular germinative cells, peripheral nuclear palisading and adjacent papillary mesenchymal cells. Follicular differentiation may also be suspected in the presence of matrical shadow (ghost) cells, and if tumour is attached to normal follicular structures. Apocrine cells have abundant eosinophilic cytoplasm and eccentric, basally located nuclei. Although the presence of decapitation secretion is deemed evidence for apocrine differentiation, this finding is considered useful but not very specific.

Thorough morphological evaluation should be performed to reach proper classification and correct diagnosis (tables 1 and 2). Benign SAT are usually multilobulated, and have symmetric and smooth borders, and uniform collections of epithelial cells, usually with no tumour necrosis or ulceration. There is usually no atypia, and the mitotic activity is generally minimal. Dense fibrotic stromal reaction occurs frequently in these tumours. Malignant features in SAT include asymmetrical growth, jagged/infiltrative borders, irregular arrangement of neoplastic cells, cytonuclear atypia and increased mitotic activity. Tumour necrosis and superficial ulcerations are commonly seen. The tumour-associated sclerotic stroma is usually diminished. The presence of these worrisome features should prompt wide excision of these tumours, with meticulous examination of all resection margins. Sentinel lymph node biopsy may be indicated in some cases; however, the role of sentinel node biopsy in malignant SAT is not yet fully defined. The patients should be closely followed up for potential regional and distant metastasis.

Table 1

 Histopathological diagnostic features to consider in the evaluation of benign skin adnexal lesions

Table 2

 Morphological patterns and useful observations in evaluating skin adnexal tumours

The need for special and/or immunohistochemical stains in the evaluation of SAT varies from one case to another, and can be used when necessary. Periodic acid Schiff (PAS) stain, with and without diastase, can be helpful to demonstrate cytoplasmic glycogen contents and stromal hyalinised basement membrane that present in certain cutaneous adnexal lesions. Hale’s colloidal iron stain for acid mucin is useful to highlight stromal mucinous degeneration in certain adnexal tumours. Prussian blue may be useful in demonstrating iron deposits within apocrine lesions. IHC and ultrastructural ancillary studies may aid in establishing the tumour differentiation, but they have limited diagnostic value and yield. Most SATs express different types of cytokeratins. Monoclonal CEA and EMA can be seen in tumours with ductal differentiation. EMA is expressed in tumours with sebaceous differentiation. GCDFP-15 and androgen receptors are seen in apocrine lesions, whereas oestrogen and progesterone receptors are seen in different sweat glands lesions and are not considered specific.


Basaloid follicular hamartoma

Basaloid follicular hamartoma (BFH) is a unique benign lesion, characterised by variable clinical presentation, and association with cutaneous and systemic diseases. It can be acquired or inherited as an autosomal dominant disease.7 The acquired BFH can present as a solitary lesion that affects middle-aged and elderly people. The lesion mostly involves the scalp and face, or can be generalised, which is characteristically associated with other clinical conditions such as myasthenia gravis, basal cell carcinoma (BCC),8 systemic lupus erythematosus and alopecia.9,10 Inherited familial BFH presents during adulthood as numerous, widely spread, small flesh-coloured or pigmented papules, and may be associated with alopecia and cystic fibrosis.11 Histologically, the lesion consists of multifocal proliferation of small basaloid cells admixed with squamous cells, forming anastomosing strands, branching cords or lobules extending from hair follicles, and embedded in loose, occasionally mucinous or densely fibrous stroma (fig 2A). Peripheral palisading of the basaloid cells, foci of keratinisation and horn cyst formation, and abortive hair follicles can be present, making BFH histologically indistinguishable from trichoepithelioma. Histological features of BFH are not always diagnostic, especially in superficial or inadequate biopsies, and should be differentiated from other cutaneous lesions with basaloid proliferation, particularly BCC. Fewer mitoses, decreased single-cell necrosis and absence of artifactual retraction are helpful discriminating features from BCC.8 It is also worth mentioning that differentiating BFH from other lesions with basaloid proliferation (such as BCC) can be difficult on frozen sections, especially during Mohs surgery, and may require an experienced pathologist to assess the margins.

Figure 2

 (A) Basaloid follicular hamartoma, (B) basaloid epidermal proliferation (BEP) overlying cutaneous fibrous histiocytoma and (C) BEP in nevus sebaceous.

Basaloid epidermal proliferation overlying dermal mesenchymal lesions

Basaloid epidermal proliferations (BEPs), morphologically resembling superficial BCC, have been described overlying cutaneous mesenchymal lesions such as fibrous histiocytoma (dermatofibroma)13 (fig 2B) and cutaneous myxomas.14 Not uncommonly, BEP is also present in the epidermis overlying NSJ (fig 2C). The pathogenesis of BEP is not clear. It is currently believed that BEP represents benign adnexal proliferation with follicular differentiation, and that it may be stimulated and maintained by the underlying proliferating mesenchymal lesion.15 Although in most cases BEPs behave in a benign fashion, and can even regress, the possibility of these lesions possessing a malignant potential is controversial.16,17 Distinguishing BEP from BCC is important because of the current differences in therapeutic approach and expected clinical outcome. In comparison to BCC, BEPs are limited to the epidermis overlying the dermal lesion, have no infiltrative growth pattern, contain sparse mitotic figures, express no or inconspicuous p53 immunoreactivity,13,18 and may occasionally contain Merkel cells (detected by anti-CK 20 IHC staining).19 Conflicting results about the immunoexpression of proliferative marker MIB-1 (Ki-67)15,17 and bcl-213,17 in BEP have been reported in the literature. Basaloid proliferations arising in hair follicles may also be seen within fibrous papules (angiofibromas) of the nose.

Trichofolliculoma, folliculosebaceous cystic hamartoma, trichodiscoma/fibrofolliculoma and other closely related lesions

Trichofolliculomas are benign hamartomatous lesions that develop at any age, and typically involve the face. It is believed that trichofolliculomas represent abortive differentiation of cutaneous pluripotent stem cells towards hair follicles. They usually present as skin-coloured, single or multiple small nodules with central epidermal ostium, in which hair emerge. Histologically, trichofolliculomas consist of centrally located, keratin-filled, unilocular or multilocular cystic cavities, usually connected to the epidermis, and lined by infundibular squamous epithelium with prominent granular layer (fig 3A). Numerous secondary and tertiary hair follicles surrounded by variable numbers of sebaceous glands bud out and branch radially from the central cavity into a fibrotic stroma, giving it a “caput medusa” appearance. Characteristically, a large number of Merkel cells can easily be identified within the tumour cells by IHC examination.20 A single case of trichofolliculoma with perineural invasion has been reported in the literature.21 Sebaceous trichofolliculoma (fig 3B) is a rare variant of trichofolliculoma, and is characterised by prominent lobules of well-differentiated sebaceous glands within the tumours, but lacking the vellus hair component.22 A differential diagnosis of sebaceous trichofolliculoma is folliculosebaceous cystic hamartoma (FSCH), which is a very rare hamartomatous lesion, histologically consisting of a prominent epithelial component characterised by folliculosebaceous proliferation with cyst-like infundibular dilatation,23,24 without connection to the overlying epidermis. Cleft-like spaces separate the epithelial component from the surrounding stroma, which is characteristically composed of dense collagenous tissue, and can contain variable amounts of mature adipose tissue, and exhibit vascular and neural proliferation. Both the lack of epidermal connection and prominent stromal component are important morphological features discriminating FSCH from trichofolliculoma.

Figure 3

 (A) Trichofolliculoma, (B) sebaceous trichofolliculoma, (C) pilar sheath acanthoma and (D) trichodiscoma/fibrofolliculoma.

Another differential diagnosis of trichofolliculoma is pilar sheath acanthoma, which is a benign follicular tumour, usually seen in adults as small single papules, mostly on the upper lip. Characteristically, it has a tiny central punctum, and lacks clinically identifiable hair structures. Histologically, it has a superficial broad invagination of cells, which often have clear (glycogenated) cytoplasm, producing a crater-like cavity (fig 3C). The lesion is associated with epidermal/infundibular and isthmic differentiation. Hence, solid lobules of basophilic and eosinophilic cells are present within the superficial dermis, similar to the epithelium of the infundibulum and isthmus, respectively. Although dilated pore of Winer might be a differential diagnosis of trichofolliculoma and pilar sheath acanthoma, it is an infundibular cyst that shows invagination of the surface epithelium, lined by relatively thin stratified squamous epithelium, and lacking isthmus differentiation.

In trichodiscoma/fibrofolliculoma (fig 3D), the lesion is characterised by dilated follicular infundibulum, from which there is proliferation of thin, trabeculated strands of basophilic infundibular epithelium within a prominent fibrous stroma. The stroma contains a large number of small vascular channels, and stromal mucinous change is often present. Multiple lesions are seen in Birt–Hogg–Dube syndrome, which is an autosomal dominant condition characterised by trichodiscomas/fibrofolliculomas, and associated with benign and malignant renal neoplasms, lung cysts and spontaneous pneumothorax.25

Trichoepithelioma and desmoplastic trichoepithelioma

Classical trichoepithelioma is a benign tumour that can occur as: (1) a sporadic, solitary lesion that can be found on any portion of hair-bearing skin, with a predilection for the head and neck region in adults, or (2) an autosomal dominant familial disorder characterised by multiple, small lesions, with a predilection for the head and neck, and upper trunk regions in adolescence. The gene associated with multiple familial trichoepithelioma (MFT) links to the short arm of chromosome 9, in which several tumour suppressor genes are located.26 Recently, mutations in the CYLD gene have been found to play a role in the pathogenesis of MFT.27,28 Histologically, trichoepithelioma is a well-circumscribed and symmetric lesion with or without connection to the epidermis. It consists predominantly of uniform basaloid cells with peripheral palisading, arranged in variably sized nests, trabeculae or cribriform patterns, and surrounded by dense stroma that contains fibroblasts resembling follicular germs and bulbs, and follicular papillae. In contrast with BCC (table 3), a major histological differential diagnosis, artefactual retraction is uncommon, and is present within the fibrotic stroma. Small horn cysts filled with keratin are usually present within the epithelial nests (fig 4). Immunohistochemically, trichoepithelioma expresses CK 5, 6, 7, 14, 17, and 15; the latter is found to be expressed in most trichoepithelioma but not in BCC.29 Positive immunostaining for bcl-2 may be helpful in differentiating TE from BCC; it tends to be diffuse in BCC, whereas it is peripheral in trichoepithelioma.30,31 In addition, CD34 is found to be positive in the stroma of trichoepithelioma, but absent in BCC.32 Trichoepithelioma and BCC also have different patterns of CD10 positivity and MIB-1 (Ki-67) labelling index.33,34 Rarely, a BCC may develop within a trichoepithelioma in which case features of both tumours may be seen in the same lesion (fig 5).

Table 3

 Useful histological and immunohistochemical features for distinguishing between basal cell carcinoma and trichoepithelioma

Figure 4

 Trichoepithelioma, with keratin cysts and tubular/eccrine differentiation (arrows).

Figure 5

 An adnexal tumour with features of trichoepithelioma on haematoxylin and eosin stain (A and B) and CD34-positive stroma (C), shown to have the immunostaining pattern of basal cell carcinoma with diffusely positive bcl-2 (D), peripheral layer positivity with CD10 (E) and increased MIB-1 labelling index (F).

Trichoadenoma is a rare benign follicular neoplasm characterised by proliferation of follicular germ cells with inconspicuous basaloid cells, predominance of keratin cysts and without hair shaft formation. In contrast with trichoadenoma, keratin cysts do not predominate in trichoepithelioma. Rare cases of malignant transformation of trichoepithelioma have been reported, in which there is an infiltrative growth pattern associated with cytological pleomorphism, mitotic activity and necrosis.35 Desmoplastic trichoepithelioma (DTE) variant occurs most commonly on the faces of young females. Histologically, it is characterised by narrow, compressed strands of basaloid germinative epithelium, with or without small horn cysts, embedded in densely sclerotic stroma within the upper dermis36 (fig 6A–C). It is important to differentiate DTE from morphoea-like BCC. The presence of horn cysts and CD34 positivity in stromal cells, and the absence of peripheral palisading, mitoses, retraction artefact and stromal expression of stromelysin-3 are useful discriminating features.32,37–39 CK 20 may identify occasional Merkel cells within DTE, which are absent in BCC.40 In comparison with syringoma, trichoepithelioma exhibits peripheral palisading, horn cyst formation, foreign body granuloma and more calcification (fig 6A–C). Syringoma is characterised by proliferation of small ducts, which are composed of two layers of cells, embedded in a sclerotic connective tissue stroma (fig 6D). There is no keratinisation. Other differential diagnoses of DTE are metastatic breast carcinoma and microcystic adnexal carcinoma; both, in comparison with DTE, are usually immunoreactant to CK 7.

Figure 6

 Desmoplastic trichoepithelioma (DTE): (A) desmoplastic stroma and giant cell reaction; (B) calcification (arrow); (C) ossification (arrow); and (D) syringoma. Note the sclerotic stroma similar to that seen in DTE.


Trichoblastoma is a benign tumour of follicular germinative epithelium, closely related to trichoepithelioma and DTE. The latter two lesions are traditionally distinguished from trichoblastoma by their characteristic morphological appearance, and prominent superficial follicular differentiation. Clinically, TB is a solitary, well-circumscribed lesion that occurs in the deep dermis and subcutaneous tissue, most commonly in elderly individuals, with a predilection to the head and neck region, trunk and extremities. It consists of nodules of cords and nests formed of solid basaloid germinative epithelial cells that exhibit peripheral palisading and brisk mitotic activity (fig 7A,B). Follicular papillae are characteristically present, and small keratin cysts may be found. Numerous melanocytes and melanin pigment are also frequently seen within the epithelial component (pigmented TB; fig 7C). The minimal intervening stromal component separates the epithelial nodules, with absent or inconspicuous cleft artefact. Trichoblastoma is the most common neoplasm that develops in association with NSJ.41,42 Merkel cells, identified by CK 20, are frequently seen in trichoblastoma. An important differential diagnosis of trichoblastoma is BCC, as the two tumours share many histological features (table 4). Trichoblastic fibroma is a morphological variant of trichoblastoma, which can present as a well-circumscribed nodular or less commonly poorly defined plaque lesion,43 and histologically consists of lobules of basaloid cells with peripheral palisading and brisk mitotic activity, with or without keratin cysts (fig 7D). It is characterised by a prominent fibromyxoid stromal component, which contains spindle and stellate fibroblasts and papillary mesenchymal bodies (primitive hair papilla formation).44 Occasionally, some lesions of trichoblastoma contain features of trichoepithelioma (such as keratin cysts and ductal differentiation), and these are referred to as trichoepitheliomatous trichoblastoma.

Table 4

 General features comparing basal cell carcinoma with trichoblastoma

Table 6

 Useful histological features for distinguishing between sebaceoma and basal cell carcinoma with sebaceous differentiation

Figure 7

 Trichoblastoma: (A) nodules of basaloid germinative epithelial cells with peripheral palisading and (B) brisk mitotic activity (arrows). (C) Pigmented trichoblastoma. (D) Trichoblastic fibroma.

Based on the current understanding of the origin of BCC as a tumour of skin adnexal structures, many authors consider trichoblastic carcinoma as a synonym for BCC. However, unlike classic BCC, the very rare trichoblastic carcinoma arising in trichoblastoma seems to have a higher potential for distant metastasis.45,46 Trichoblastic carcinoma is characterised by its “bottom heavy” configuration and the restricted tumour stroma, and may infiltrate into underlying muscles.


Trichilemmomas are benign tumours that arise from the outer root sheath of the hair follicle, mainly of the bulb region. They usually present as a solitary small skin-coloured papule, or verrucous lesion, and have a predilection for faces of older adults, particularly over the nose. Multiple facial trichilemmomas characteristically occur in association with Cowden syndrome (multiple hamartoma syndrome).47 This genodermatosis is an autosomal dominant disorder, characterised by multiple hamartomas in the skin, breast, thyroid, gastrointestinal tract, endometrium and brain, and is associated with significantly increased risk of malignancy in these organs. Histologically, trichilemmoma is a well-circumscribed and symmetric lesion, with broad connection with the epidermis, which is hyperplastic and may show verruca vulgaris-like changes; however, this connection may not be identified in some cases. The prototypical early lesion shows hyperplasia of the infundibular epithelium, with differentiation towards the outer root sheath (fig 8A,B). In fully developed lesions (fig 8C), trichilemmoma consists of lobules or plates of uniform cells with glycogenated, periodic acid Schiff (PAS)-positive/diastase-sensitive clear cytoplasm. The cells at the periphery of tumour lobules are more basophilic, arranged in a palisading pattern, and surrounded by thick, hyaline, PAS-positive/diastase-resistant basement membrane material. Foci of keratin microcysts and squamous eddies may be seen. Trichilemmoma should be distinguished from inverted follicular keratosis, clear BCC and hidroacanthoma simplex (HAS; epidermal poroma). The presence of palisading and hyaline basement membrane material, and the absence of ductal differentiation are helpful features in separating trichilemmoma from HAS. Inverted follicular keratosis is characterised by squamous and basaloid epithelial expansion of the infundibular portion of the hair follicle, associated with squamous eddies similar to irritated seborrhoeic keratosis. Desmoplastic trichilemmoma is a histological variant characterised by the presence of strands of tumour cells, entrapped in dense, hypocellular and fibrotic dermis (fig 8D,E), and resembling desmoplastic BCC and squamous cell carcinoma (SCC).48,49 The presence of features of conventional trichilemmoma at the periphery of a desmoplastic lesion, presence of PAS-positive/diastase-resistant and Alcian blue-positive material in dermal stroma, and the immunohistochemical expression of CD34 are important features that favour the diagnosis of desmoplastic trichilemmoma.50 Very rare cases of trichilemmal carcinoma (fig 8F) have been described.51,52 These cases exhibit only trichilemmal differentiation, and should not be confused with BCC, particularly in a superficial biopsy.

Figure 8

 Trichilemmoma. (A) An early lesion (B) with verruca-like changes. (C) An established lesion. (D,E) Desmoplastic trichilemmoma. (F) Trichilemmal carcinoma, with atypia and tumour necrosis.

Proliferating trichilemmal cyst (pilar tumour)

Proliferating trichilemmal cyst (PTC) is a benign lesion, most commonly occurring on the scalps of elderly women. PTC presents as a solitary nodulocystic lesion, usually ranging in size from 1 to 10 cm in diameter, and very rarely reaching up to 25 cm in size.53 Occasional patients may develop multiple tumours.54 PTC is believed to originate from a pre-existing trichilemmal cyst. Histologically, PTC exhibits differentiation towards the follicular outer root sheath, consists of well-defined lobulated, solid and cystic mass of proliferating squamous epithelium, surrounded by thick hyalinised basement membrane, and involves the deep dermis and subcutaneous tissue (fig 9A). Extension of epithelial growths into the lumen, central trichilemmal keratinisation, and peripheral palisading of small basaloid cells may be seen. The squamous cells exhibit bland or mildly atypical cytonuclear features, and inconspicuous, basally located mitotic activity. Individual cell keratinisation and squamous eddies are characteristic findings. Focal necrosis and calcification can be present in large tumours. Stromal reactive multinucleated giant cell reaction is frequently present (fig 9B). Foci of residual, simple pilar cyst(s) may be identified. It is important to distinguish PTC from SCC (box 2). Malignant transformation in PTC is uncommon. Desmoplasia and/or squamous budding into the tumour stroma within the lesion should not be confused with SCC (fig 9C), as it represents pseudoinvasion. Features of malignant PTC include a combination of non-scalp locations, size >5 cm, recent rapid increase in size, infiltrative growth pattern (fig 9D), significant cytonuclear atypia and brisk mitotic activity.55 Particularly large and long-standing lesions can potentially recur if not adequately excised by wide local excision,56 and involvement of lymph nodes and distant metastases are reported.57,58

Figure 9

 Pilar tumour (A) characteristic lobulation, (B) trichilemmal keratinisation and associated cholesterol clefts and foreign body type giant cell reaction, (C) keratin pearls and budding into the stroma within the tumour should be recognised as pseudoinvasion, compared with true malignant change, which is seen at the infiltrative peripheral margins (D).

Box 2 Histological features distinguishing proliferating trichilemmal cyst from squamous cell carcinoma

  • Presence of pre-existing simple pilar cyst

  • Good circumscription

  • Smooth peripheral margins

  • Trichilemmal keratinisation

  • Absent or inconspicuous cytonuclear atypia and minimal mitotic activity

  • Presence of peripheral palisading

  • Hyaline basement membrane

Pilomatricoma (pilomatrixoma)

Pilomatricoma or calcifying epithelioma of Malherbe is a benign dermal and/or subcutaneous tumour, most commonly affecting children and adolescents; it is slightly more common in females. Pilomatricoma usually occurs as a solitary firm lesion with predilection for head and neck, and upper extremities. Rare cases of multiple pilomatricoma are associated with different conditions such as myotonic dystrophy,59 Rubinstein–Taybi syndrome60 and Turner syndrome.61 Activation mutations of β-catenin are found to contribute to tumorigenesis of pilomatricoma.62 Classic pilomatricoma is readily identified by light microscopic examination, which shows a well-circumscribed nodular lesion in the dermis and/or subcutis, surrounded by fibrous stroma (fig 10A). Early lesions are usually cystic, and consist of mitotically active, uniform, basaloid cells lining the cystic cavity, and contiguously transformed into pale eosinophilic anucleated shadow/ghost cells admixed with keratin (fig 10B,C). The shadow cells represent faulty attempts to produce a hair shaft. Older pilomatricomas become solid, and are characterised by prominent shadow cell component, keratin debris, secondary multinucleated giant cells (fig 10D) and dystrophic calcification. Less commonly, bone formation with extramedullary haematopoiesis may also occur.63 Perforating pilomatricoma is a histological variant characterised by transepidermal elimination of shadow cells and keratin. Proliferating pilomatricoma is another variant, occurring in the middle-aged and elderly, and is characterised by prominent lobulation and proliferation of basaloid cells, and inconspicuous foci of shadow cells.

Figure 10

 Pilomatricoma: (A) a multilobulated mass in fibrous stroma reaching the subcutis; (B) basaloid and ghost cells; (C) mitoses in an early lesion; and (D) giant cell reaction in an established lesion.

Pilomatrix carcinoma is very rare, and mostly occurs in head and neck, upper limbs, and buttocks. It is locally aggressive, with great tendency for local recurrence, and, potentially, can metastasise to regional lymph nodes and distant organs.64,65 Histologically, it differs from pilomatricoma in asymmetrical growth pattern, poor circumscription, significant cytonuclear atypia, markedly increased mitotic activity and infiltration to the adjacent tissue. Tumour necrosis, calcification and ossification may also be seen. The presence of lymphovascular and perineural invasion, extension into the bone, and distant metastasis are definitive features of malignancy.


Sebaceous hyperplasia, sebaceous adenoma, sebaceoma and sebaceous carcinoma

Sebaceous adenoma, and, more frequently, sebaceous hyperplasia are common benign skin adnexal lesions that principally affect middle-aged and elderly individuals. They are usually solitary, and present as a small, yellow, lobulated papule with a predilection to the forehead and cheeks. Multiple sebaceous lesions (hyperplasia, adenoma, sebaceoma and carcinoma) can occur in association with Muir–Torre syndrome, an autosomal dominantly inherited disorder characterised by visceral malignancies (colorectal, upper gastrointestinal, endometrial and urological malignant neoplasms), and tumours of sebaceous glands or keratoacanthoma.66 Sebaceous hyperplasia represents a single enlarged sebaceous gland and shows five or more lobules of bland, immature sebocytes that open into a single dilated follicular infundibulum (fig 11A). The peripheries of the lobules have one or two cell layers of basaloid germinative cells. Sebaceous hyperplasia should be differentiated from rhinophyma, in which the sebaceous gland hyperplasia is associated with perifollicular inflammation and dermal fibrosis, and not confined to one focal area.

Figure 11

 Benign sebaceous neoplasms: (A) sebaceous hyperplasia; (B) sebaceous adenoma; (C) sebaceoma; (D) peripheral palisading and sebaceous ductal differentiation in sebaceoma (arrow); (E) sebaceoma with squamous metaplasia (arrow).

Sebaceous adenoma is a well-circumscribed dermal nodule, histologically formed of lobules of predominate mature, bland sebaceous cells, and peripherally located in one or two layers of germinal basaloid epithelial cells (fig 11B). There is no central draining duct. Sebaceoma (formerly known as sebaceous epithelioma) is a histological variant of SA, in which the basaloid epithelial cells constitute ⩾50% of the SA (fig 11C). It is characterised by variably sized lobules, which are composed predominantly of basaloid cells, admixed with single or clustered mature sebaceous cells, and exhibit sebaceous ductal differentiation (fig 11D). Foci of squamous metaplasia may rarely be seen (fig 11E). Some authors believe that sebaceoma is synonymous with BCC with sebaceous differentiation. However, we agree with others67 that these two entities should be separate, as there are well-defined histological differences (table 5). A verrucous variant of sebaceoma characterised by connection with a hyperplastic infundibulum in the upper portion of the lesion, prominent granular layer and basosquamous differentiation, similar to those seen in seborrhoeic keratosis, has been described.68 Superficial epithelioma with sebaceous differentiation is a rare slow-growing tumour, characterised by anastomosing basaloid cells mixed with sebaceous cells broadly attached to the overlying surface epithelium. As many of these tumours also have peripheral palisading, many authors believe the term to be synonymous with superficial BCC with sebaceous differentiation.

Sebaceous carcinoma most frequently affects the eyelid (ocular, palpebral sebaceous carcinoma), and has potential for local recurrence and distant metastases. Sebaceous carcinoma of the eyelid is derived from the modified sebaceous glands (Meibomian glands or glands of Zeis). When associated with Muir–Torre syndrome, sebaceous carcinoma appears to have a better prognosis. Histologically, sebaceous carcinoma differs from sebaceous adenoma and sebaceoma in its asymmetry, poor circumscription, infiltrative growth pattern, and preponderance of pleomorphic, basaloid cells that are arranged in solid sheets, and shows marked cytonuclear atypia and high mitotic activity (fig 12A,B). Scattered sebocytes are often present within the basaloid tumour mass. Peripheral palisading and artefactual clefting are absent. The presence of tumour necrosis denotes bad prognosis68 (fig 12C). Superficial spread resembling Bowen-like disease and/or pagetoid pattern, involving the overlying epidermis, particularly in ocular sebaceous carcinoma may occasionally be identified.69 IHC shows sebaceous carcinoma to be immunopositive for AE1/AE3 cytokeratin, LMWCK, EMA, anti-breast carcinoma associated antigen-225 antibody (CU18), anti-CA 15.3 antibody, and androgen receptor protein, and immunonegative for CEA, S100 protein and GCDFP-15.70,71 Immature and mature neoplastic sebocytes can also express CK 14.72

Figure 12

 Sebaceous carcinoma: (A) lobulated mass of basaloid cells and inconspicuous sebocytes, (B) cellular atypia and brisk mitotic activity, (C) tumour necrosis and mitoses (arrow) and (D) focal spindle cell differentiation.

Different histological variants of sebaceous carcinoma are recognised, the most important being the basaloid, spindle cell, squamoid and dedifferentiated (pleomorphic) variants. Basaloid sebaceous carcinoma is composed of small basaloid cells, with palisading at the periphery of the epithelial lobules, and inconspicuous sebocytes. The spindle cell variant is predominantly formed of spindle cells with foci of squamous metaplasia (fig 12D). In these cases, EMA can be used to identify sebocytes; LMWK and D2-40 can help as they may be positive, but they are not specific.

Nevus sebaceous of Jadassohn

This is a benign congenital malformation, usually presenting at birth as solitary yellowish, waxy hairless plaques, or linear lesions, and as verrucous nodules in adults. It most commonly affects the head and neck region, with predilection to the scalp. NSJ, particularly if multiple, can be associated with epidermal nevus syndrome, epidermal hamartomas and extracutaneous abnormalities, principally involving the brain, eye and skeletal system.73 Histologically, NSJ is a hamartomatous lesion that represents mature elements of all lines of adnexal differentiation, and shows a wide range of morphological features, depending on the lesion’s growth phase. Early lesions show multiple foci of lobules of mature sebaceous glands, and a small number of disorganised eccrine ducts and apocrine sweat glands. In adults, as the lesion progresses, the sebaceous lobules in the well-developed lesions become prominent, and poorly formed follicular units caused by proliferation of basaloid follicular germinal epithelium can be seen. Characteristically, lobules of heterotropic/ectopic apocrine glands are identified in the reticular dermis. The overlying epidermis shows papillomatous/verrucous hyperplasia, and the sebaceous lobules are numerous, irregular and directly open into the epidermis (fig 13A). The term “organoid nevus” has been used to describe lesions which show overlapping features of NSJ and epidermal nevi. A variety of lesions have been described in association with NSJ; the incidence of these lesions increases with age, particularly after puberty. These include benign adnexal tumours such as trichoblastoma, SCAP (fig 13B), trichilemmoma, BEP (fig 2C), sebaceoma, nodular hidradenoma, hidrocystoma and eccrine poroma.41,42 Other lesions include seborrhoeic keratosis, leiomyoma, melanocytic nevi, and viral warts. Trichoblastoma and SCAP are the most common benign tumours encountered in NSJ. Malignant cutaneous neoplasms are less commonly seen, and include BCC, and, to a lesser extent, SCC,74 trichilemmal carcinoma,75 sebaceous carcinoma,76 porocarcinoma77 and apocrine carcinoma.78 In a recent study of 596 cases of NSJ, malignancies were encountered in <1% of cases, whereas benign tumours were identified in 13.6% of cases of NSJ.42 In many cases, different tumours coexist within an individual NSJ.74,75,79 Allelic deletions of the human homologue of the Drosophila-patched gene are described in NSJ,80 and this may account for the development of aggressive malignant tumours within the lesions. In general, prophylactic and complete excision is advised in all cases of NSJ.

Figure 13

 (A) Nevus sebaceous of Jadassohn revealing prominent sebaceous glands opening directly to the surface, with immature hair follicles and heterotopic apocrine glands; (B) syringocystadenoma papilliferum on the right side, with basaloid proliferations (arrow), arising in a nevus sebaceous.

Take-home messages

  • Skin adnexal tumours are usually classified according to the predominant morphological component.

  • Most skin adnexal tumours are benign, but some of these tumours might be markers for syndromes associated with internal malignancies.

  • Morphological evaluation is very important in evaluating skin adnexal tumours, and special and/or immunohistochemical stains may occasionally serve as ancillary tools.

  • Follicular differentiation is characterised by the presence of proliferation of basaloid bulbar germinative cells, peripheral nuclear palisading and adjacent papillary mesenchymal cells. The presence of shadow (ghost) cells and tumour attachment to normal follicular structures are other features.

  • Sebaceous differentiation is indicated by the presence of cells with coarsely vacuolated cytoplasm and starry nuclei (mulberry cells), which are characteristically positive epithelial membrane antigens.



  • Published Online First 1 August 2006

  • Competing interests: None declared.