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Complex analysis of cyclin D1 expression in mantle cell lymphoma: two cyclin D1-negative cases detected
  1. L Stefancikova1,2,
  2. M Moulis1,3,
  3. P Fabian4,
  4. I Falkova1,
  5. I Vasova5,
  6. L Kren1,
  7. J Macak1,
  8. J Smardova1,2,3
  1. 1Department of Pathology, University Hospital Brno. Jihlavska 20, 625 00 Brno, Czech Republic
  2. 2Department of Experimental Biology, Faculty of Science, Masaryk University, Kotlarska 2, 611 37 Brno, Czech Republic
  3. 3Faculty of Medicine, Masaryk University, Komenskeho namesti 2, 662 43 Brno, Czech Republic
  4. 4Department of Pathology, Masaryk Memorial Cancer Institute, Zluty kopec 7, 656 53 Brno, Czech Republic
  5. 5Department of Hematooncology, University Hospital Brno. Jihlavska 20, 625 00 Brno, Czech Republic
  1. Correspondence to Dr J Smardova, Department of Pathology, University Hospital, Jihlavska 20, 625 00 Brno, Czech Republic; janasmarda{at}seznam.cz

Abstract

Background and Aim: The cytogenetic and diagnostic hallmark of mantle cell lymphoma (MCL) is translocation t(11;14)(q13;q32), resulting in overexpression of cyclin D1. Cyclin D1 expression was analysed in 32 cases of MCL.

Methods: The t(11;14) translocation was detected by fluorescence in situ hybridisation, level of cyclin D1 mRNA by competitive RT-PCR, and level of cyclin D1 and D2 proteins by immunohistochemistry and/or immunoblotting.

Results: In 30 cases, the presence of translocation t(11;14), a high level of cyclin D1 mRNA, and a high level of the cyclin D1 protein were confirmed. Two cyclin D1-negative cases overexpressing cyclin D2 were detected by immunoblotting.

Conclusions: There are rare cyclin D1-negative cases of MCL overexpressing cyclin D2. Anti-cyclin D1 antibodies with low specificity can bind both cyclin D1 and cyclin D2, thus providing false cyclin D1-positive signals in immunohistochemical analysis.

https://doi.org/10.1136/jcp.2008.063701

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    Footnotes

    • Funding This work was supported by grants NR/9305-3 of IGA of Ministry of Health of the Czech Republic, MSM0021622415 of Ministry of Education, Youth and Sports of the Czech Republic, and 204/08/H054 of Grant Agency of the Czech Republic.

    • Competing interests None declared.

    • Ethics approval Ethics approval was obtained from the Ethics Committee of University Hospital Brno.

    • Provenance and peer review Not commissioned; externally peer reviewed.