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Acute enterovirus infection followed by myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) and viral persistence
  1. J Chia1,
  2. A Chia1,
  3. M Voeller2,
  4. T Lee3,
  5. R Chang4
  1. 1EV Med Research, Torrance, California, USA
  2. 2Department of Microbiology, Torrance Memorial Medical Center, Torrance, California, USA
  3. 3South Bay Gastroenterology Medical Group, Torrance, California, USA
  4. 4Peninsula Pulmonary Institute, Torrance, California, USA
  1. Correspondence to John Chia, 25332 Narbonne Ave no. 170, Lomita, CA 90717, USA; evmed{at}


Aims Enteroviruses are well-known causes of acute respiratory and/or gastrointestinal infections and non-specific flu-like illness. Although enterovirus protein, RNA and non-cytopathic viruses have been demonstrated in the stomach biopsies of patients with myalgia encephalomyelitis/chronic fatigue syndrome (ME/CFS), causality for chronic diseases is difficult to establish without having well-documented cases of acute enterovirus infections. The aim of this study was to link acute enteroviral infection to viral persistence in patients with ME/CFS.

Method Patients admitted to the hospital with acute febrile illnesses were screened for enteroviral infections. Acutely infected patients were followed longitudinally, and those who developed symptoms of ME/CFS underwent oesophagogastroduodenoscopy and biopsies of the antrum to document viral persistence by immunoperoxidase staining for viral protein and viral RNA assay.

Results Three representative patients with different manifestations of acute enterovirus infections progressed to have chronic symptoms of ME/CFS. Persistent viral infection was demonstrated in the antrum years later.

Conclusion After acute infections, enteroviruses can persist in patients resulting in manifestation of ME/CFS. Chronic enterovirus infection in an immunocompetent host may be an example of a stalemate between attenuated, intracellular viruses and an ineffective immune response.

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  • Competing interests EV Med Research has a patent for using the immunoperoxidase test to diagnose ME/CFS.

  • Patient consent Obtained.

  • Provenance and peer review Not commissioned; externally peer reviewed.