Background Clarithromycin (CH) and metronidazole (MZ) are routinely used in Helicobacter pylori treatment regimes. Recently, treatment with these antibiotics has been reported to fail in >30% of patients due to increasing resistance. The common chemosusceptibility methods are cumbersome and take up to 14 days. Chemosusceptibility of H pylori is therefore rarely checked.
Aim To develop a rapid susceptibility test (RST) for H pylori.
Methods Preliminary experiments were performed to find a broth medium able to support the growth of H pylori in 20 h and a reliable detection method to quickly detect the amount of H pylori grown in the media. A mixture of different components was the best broth medium; ELISA was chosen as the detection technique. Performance of the new RST was compared to a gold standard (break point agar dilution method). 200 consecutive patients were tested for H pylori infection and chemosusceptibility to CH and MZ by the gold standard and RST.
Results 111 patients were infected with H pylori. 105 of these were positive by both RST and the gold standard culture method; 75/105 and 68/105 strains were susceptible to CH and MZ, respectively. RST misdiagnosed 2 strains of CH and 10 strains of MZ.
Conclusions A new simple and rapid chemosusceptibility test for H pylori has been validated, which is simple to perform and reduces the procedure time from 6–14 days to 24 h.
- drug resistance
- helicobacter pylori
- helicobacter pylori - assessment
- helicobacter pylori - susceptibility
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This paper refers to the patent pending application ‘A chemosusceptibility rapid test for Helicobacter pylori’ PCT/IT2007/000270; applicant: Alma Mater Studiorum, University of Bologna. For more detailed information about the invention, please refer to the Knowledge Transfer Office at: +390512099439; e-mail: http://www.unibo.it/services for companies.; website:
Funding This work was financially partially supported by the University of Bologna.
Competing interests None.
Provenance and peer review Not commissioned; externally peer reviewed.