Article Text
Abstract
Aims Peripheral T cell lymphoma not otherwise specified (PTCL/NOS) is the commonest PTCL subtype. Recently, proliferation pathways have been found to be commonly altered in PTCL/NOS. CDKN1B/p27, a critical regulator of cell cycle and proliferation, has been suggested to be involved in T cell lymphomagenesis. This study aimed to evaluate the possible occurrence of CDKN1B/p27 aberrations in PTCL/NOS.
Methods CDKN1B/p27 expression at RNA and protein level by DNA and tissue microarrays, in 28 and 98 cases, respectively, was studied. Additionally, direct sequencing of CDKN1B in 81 PTCL/NOS was performed.
Results CDKN1B mRNA was similarly expressed in PTCL/NOS and normal T lymphocytes. In addition, structural abnormalities were not found; these included mutations and deletions in any exons, exon–intron junctions or regulatory regions. Furthermore, physiological expression of p27 in neoplastic cells was demonstrated by immunohistochemistry; this was mutually exclusive with Ki-67, as expected when the system is intact. Consistently, the expression of other molecules that are functionally related to CDKN1B/p27 in controlling cell cycle (including CCNE1) did not appear to be affected at either the mRNA or protein level. Finally, it was found that p27 expression was not significantly related with overall survival.
Conclusion CDKN1B/p27 aberrations seem to be uncommon in PTCL/NOS pathogenesis.
- CDKN1B/p27
- gene sequencing
- haematopathology
- immunohistochemistry
- lymphoma
- microarray
- molecular genetics
- peripheral T cell lymphoma
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Supplementary materials
Web Only Data JCP.2010.083832
Footnotes
Stefano A Pileri and Pier Paolo Piccaluga contributed equally to this work.
Funding This work was supported by Centro Interdipartimentale per la Ricerca sul Cancro ‘G. Prodi’, Bologn AIL, AIRC (IG4987 and IG10007), FIRB (Professor Pileri and Professor Zinzani), RFO (Professor Pileri, Professor Zinzani, Dr Piccaluga), Fondazione Cassa di Risparmio in Bologna, Fondazione della Banca del Monte e Ravenna, Progetto Strategico di Ateneo 2006 (Professor Pileri and Dr Piccaluga).
Competing interests None.
Ethics approval This study was conducted with the approval of the ethics committee of S. Orsola-Malpighi Hospital, University of Bologna, Bologna, Italy.
Provenance and peer review Not commissioned; externally peer reviewed.