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Collagen triple helix repeat containing 1 protein, periostin and versican in primary and metastatic breast cancer: an immunohistochemical study
  1. Gvantsa Kharaishvili1,
  2. Magdalena Cizkova2,
  3. Katerina Bouchalova2,
  4. Giorgi Mgebrishvili1,
  5. Zdenek Kolar1,
  6. Jan Bouchal1
  1. 1Laboratory of Molecular Pathology and Department of Clinical and Molecular Pathology
  2. 2Laboratory of Experimental Medicine, Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University and University Hospital, Olomouc, Czech Republic
  1. Correspondence to Dr Jan Bouchal, Laboratory of Molecular Pathology, Hněvotínská 3, 77515 Olomouc, Czech Republic; jan.bouchal{at}


Background Collagen triple helix repeat containing 1 (CTHRC1) affects Wnt signalling, collagen deposition and bone formation. It is an extracellular matrix protein which is also abnormally expressed in the tumour microenvironment. CTHRC1 has not been studied in breast cancer by immunohistochemistry.

Aims To examine expression of CTHRC1 together with periostin and versican in breast cancer patients and investigate its association with clinicopathological characteristics.

Methods The formalin-fixed paraffin-embedded tissues of 173 invasive carcinomas (classified into WHO histotypes and luminal, triple negative and Her2 subtypes), as well as normal tissues, precursor lesions and metastatic lymph nodes were stained by relevant antibodies, assessed semiquantitatively by histoscore and statistically evaluated.

Results Expression of CTHRC1, versican and periostin was significantly higher in breast cancer than in normal tissue or precursor lesions. CTHRC1 stromal expression was enhanced in triple negative cases and also in patients with bone metastasis. Periostin expression was high in primary tumours, in particular triple negative ones, and also in their lymph node metastases. Cox regression analysis showed that in patients with high periostin, the risk of bone metastases increased with increased CTHRC1 expression.

Conclusions CTHRC1 and periostin play important roles in breast cancer progression. These preliminary results show that combined evaluation of CTHRC1 and periostin could serve as a potential marker for breast cancer bone metastasis; the other observations contribute to the description of the tumour microenvironment, with implications for lymph node and bone metastasis.

  • CTHRC1
  • periostin
  • versican
  • breast cancer
  • immunohistochemistry
  • prostate
  • molecular pathology

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  • Funding This work was supported by grants NS 9956-4 from the Czech Ministry of Health and MSM 6198959216 from the Czech Ministry of Education, and EU infrastructure support CZ.1.05/2.1.00/01.0030. GK was also supported by LF_2010_006.

  • Competing interests None declared.

  • Ethics approval The study was approved by the ethics committee of the Medical Faculty and University Hospital Olomouc.

  • Provenance and peer review Not commissioned; externally peer reviewed.