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Detection of HER2 and Topo 2 in breast cancers: comparison between MLPA and FISH approaches
  1. Sara Bravaccini1,
  2. Claudia Rengucci1,
  3. Laura Medri2,
  4. Wainer Zoli1,
  5. Rosella Silvestrini1,
  6. Dino Amadori1
  1. 1Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (I.R.S.T.), Meldola, Italy
  2. 2Pathology Unit, Morgagni-Pierantoni Hospital, Forli, Italy
  1. Correspondence to Dr Sara Bravaccini, Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (I.R.S.T.), Via P. Maroncelli 40, 47014 Meldola, FC, Italy; s.bravaccini{at}


A significant proportion of breast cancers with HER2 amplification show simultaneous amplification or deletion of Topo 2. Amplification of Topo 2 may lead to the overexpression of the Topo 2 protein and ultimately to hypersensitivity to Topo 2 inhibitors. HER2 and Topo 2 gene status in breast cancer patients has been determined in several studies using immunohistochemistry, florescence in situ hybridisation (FISH) and multiplex ligation-dependent probe amplification (MLPA). Although comparisons of FISH and MLPA have been reported for HER2, it is believed that there are no similar studies for Topo 2. In this study, HER2 and Topo 2 were analysed by MLPA and FISH. There was a high agreement between the two approaches, although MLPA was easier to perform and cheaper than FISH. In conclusion, MLPA is a fast and accurate quantitative method to detect HER2 and Topo 2 amplification, and could be considered a good alternative to FISH.

  • HER2
  • Topo 2
  • MLPA
  • FISH
  • breast cancer
  • cell biology
  • molecular biology
  • CERB 2
  • tumour biology
  • cancer
  • cancer research

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  • Competing interests None declared.

  • Ethics approval Ethics approval was provided by the local ethical committee.

  • Provenance and peer review Not commissioned; externally peer reviewed.