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Epidermal growth factor receptor mutations in malignant pleural and peritoneal mesothelioma
  1. Yasunori Enomoto1,
  2. Takahiko Kasai1,
  3. Maiko Takeda1,
  4. Masato Takano1,
  5. Kohei Morita1,
  6. Eiji Kadota2,
  7. Norishige Iizuka2,
  8. Hiroshi Maruyama3,
  9. Joji Haratake4,
  10. Yu Kojima5,
  11. Naoya Ikeda5,
  12. Naoki Inatsugi6,
  13. Akitaka Nonomura1
  1. 1Department of Diagnostic Pathology, Nara Medical University School of Medicine, Nara, Japan
  2. 2Department of Laboratory Medicine and Pathology, Kishiwada City Hospital, Osaka, Japan
  3. 3Department of Pathology, Hoshigaoka Koseinenkin Hospital, Osaka, Japan
  4. 4Department of Diagnostic Pathology, Saiseikai Yahata General Hospital, Yahata, Fukuoka, Japan
  5. 5Department of Surgery, Nara Prefectural Mimuro Hospital, Nara, Japan
  6. 6Department of Surgery, Kenseikai Dongo Hospital, Nara, Japan
  1. Correspondence to Dr Takahiko Kasai, Department of Diagnostic Pathology, Nara Medical University School of Medicine, Shijo-cho 840, Kashihara, Nara 634-8521, Japan; kasai{at}


Background Epidermal growth factor receptor (EGFR) gene mutation at the kinase domain and EGFR gene amplification are reported to be predictors of the response to EGFR tyrosine kinase inhibitors in lung cancer cases. In malignant mesothelioma (MM), the role of EGFR is less clear.

Methods Thirty-eight MM specimens were submitted to EGFR mutation evaluation, and compared with the results of immunohistochemical staining and fluorescence in situ hybridization (FISH) analysis. DNA was extracted from paraffin blocks and PCR was performed to amplify exon regions 18–21 of the EGFR gene. Direct sequencing of the purified PCR products was performed.

Results Five EGFR missense mutations were detected in six of the 38 patients (16%); two of these mutations were novel, two were originally detected in non-small cell lung carcinoma, and one resembled a location previously noted for malignant peritoneal mesothelioma.

Conclusion As far as the authors are aware there has been no report of the EGFR mutation of MM in Japanese cases, but in this study EGFR missense mutations were detected in some cases. EGFR mutation results were not related to immunohistochemical and FISH analysis.

  • Cancer research
  • colon
  • epidermal growth factor receptor
  • FISH
  • gastric pathology
  • gene copy number gain
  • haematopathology
  • histopathology
  • immunohistochemistry
  • malignant mesothelioma
  • malignant tumours
  • mutation
  • PCR
  • surgical pathology

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  • Competing interests None.

  • Patient consent Obtained.

  • Ethics approval Ethics approval was provided by the ethics committee of Nara Medical University.

  • Provenance and peer review Not commissioned; externally peer reviewed.