Aims Neoadjuvant chemoradiation therapy (CRT) is an increasingly used therapeutic strategy for rectal cancer. Clinically, it remains a major challenge to predict therapeutic response and patient outcome after CRT. Rsf-1 (HBXAP), a novel nuclear protein with histone chaperon function, mediates ATPase-dependent chromatin remodelling and confers tumour aggressiveness and predicts therapeutic response in certain carcinomas. However, the expression of Rsf-1 has never been reported in rectal cancer. This study examined the predictive and prognostic impacts of Rsf-1 expression in patients with rectal cancer following neoadjuvant CRT.
Methods Rsf-1 immunoexpression was retrospectively assessed for pre-treatment biopsies of 172 rectal cancer patients without initial distant metastasis. All of them were treated with neoadjuvant CRT followed by surgery. The results were correlated with the clinicopathological features, therapeutic response, tumour regression grade and metastasis-free survival (MeFS), local recurrent-free survival and disease-specific survival.
Results Present in 82 cases (47.7%), high-expression of Rsf-1 was associated with advanced pre-treatment tumour status (T3, T4, p=0.020), advanced post-treatment tumour status (T3, T4, p<0.001) and inferior tumour regression grade (p=0.028). Of note, high-expression of Rsf-1 emerged as an adverse prognosticator for diseases-specific survival (p=0.0092) and significantly predicted worse MeFS (p=0.0006). Moreover, high-expression of Rsf-1 also remained prognostic independent for worse MeFS (HR 2.834; p=0.0214).
Conclusions High-expression of Rsf-1 is associated with poor therapeutic response and adverse outcome in rectal cancer patients treated with neoadjuvant CRT, which confers tumour aggressiveness and therapeutic resistance through chromatin remodelling and represents a potential prognostic biomarker in rectal cancer.
- rectal cancer
- neoadjuvant chemoradiation
- molecular oncology
- molecular pathology
- molecular genetics
- tumour biology
- tumour markers
- cancer research
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Additional supplementary materials are published online only. To view these files please visit the journal online (http://jcp.bmj.com).
CYL and YFT contributed equally to this work.
Funding This work was supported in part by grants from Chi-Mei Medical Center (CMFHR10044) and Department of Health, Taiwan (DOH99-TD-C-111-004).
Competing interests None.
Patient consent The current study used tumour materials from the Biobank of Chi-Mei Medical Center. As a rule, all tumour samples are collected only when patient consent is completed. Once being enrolled, the samples are disconnected with identifiable private information and thus no more patient consent is needed.
Ethics approval The institutional review board had approved procurement of formalin-fixed tissue of 172 LARC patients for this study (IRB 10009-L04).
Provenance and peer review Not commissioned; externally peer reviewed.