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Original article
Circulating cell-free DNA in serum as a biomarker of colorectal cancer
  1. Benisio Ferreira da Silva Filho1,
  2. Ana Pavla Almeida Diniz Gurgel2,
  3. Manoel Álvaro de Freitas Lins Neto3,
  4. Dalmo Almeida de Azevedo1,
  5. Antonio Carlos de Freitas2,
  6. Jacinto da Costa Silva Neto4,
  7. Luiz Antonio Ferreira Silva1
  1. 1Departament of Genetics, Institute of Biological Sciences and Health, Federal University of Alagoas, Maceió, Brazil
  2. 2Department of Genetics, Federal University of Pernambuco, Recife, Brazil
  3. 3Faculty of Medicine, Federal University of Alagoas, Maceió, Brazil
  4. 4Department of Histology and Embriology, Federal University of Pernambuco, Recife, Brazil
  1. Correspondence to Dr Jacinto da Costa Silva Neto, Department of Histology and Embriology, Federal University of Pernambuco, Cidade Universitária, Recife, Pernambuco CEP 50.670-901, Brazil; Jacintocosta{at}hotmail.com

Abstract

Aims The aim of this study was to report circulating cell-free DNA using ALU247 and ALU247/ALU115 biomarkers in serum of operated and non-operated patients with colorectal cancer (CRC).

Methods To undertake this, 90 blood samples were collected, including 30 samples from healthy volunteers; 27 samples from CRC non-operated patients and 33 samples from CRC-operated patients. Circulating cell-free DNA was verified through quantitative real-time PCR (qPCR) using ALU115 and ALU247 primers.

Results With regard to the ALU115-qPCR biomarker, the increased levels of circulating cell-free DNA in serum of non-operated patients were significant when compared with control (p<0.05). Moreover, levels of ALU247-qPCR biomarker were statistically significant between non-operated versus operated and non-operated versus control groups (p=0.000). With regard to the ALU247/115-qPCR biomarker, significant differences were observed between control versus non-operated patients (p=0.019), operated versus non-operated patients (p=0.005) and control versus operated patients (p=0.043).

Conclusions Thus, the ALU247 and ALU247/ALU115-qPCR biomarkers may be important in detecting and monitoring CRC patients in both early and late stages.

  • Colorectal Cancer
  • Molecular Pathology
  • PCR

http://dx.doi.org/10.1136/jclinpath-2013-201521

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