Article Text

Download PDFPDF
Ethylenediaminetetraacetic acid-dependent pseudomacrocytosis
  1. Jose Manuel Vagace1,
  2. Miguel Ángel Rodriguez2,
  3. María Dolores de la Maya1,
  4. Guillermo Gervasini3
  1. 1Department of Pediatric Hematology, Materno Infantil Hospital, Badajoz, Spain
  2. 2Service of Immunohematology, Transfusion Center, Madrid, Spain
  3. 3Department of Surgical & Medical Therapeutics, Division of Pharmacology, University of Extremadura, Badajoz, Spain
  1. Correspondence to Dr Jose Manuel Vagace, Department of Pediatric Hematology, Materno Infantil Hospital, C/ La Violeta 4, Badajoz 06010, Spain;  jvagacev{at}


We investigated the case of a 14-year-old girl with an ethylenediaminetetraacetic acid (EDTA)-dependent haemagglutination detected by macrocytosis, which was only evident by an abnormal red blood cell (RBC) population in the histogram. Investigations included haemograms with different anticoagulants and experimental conditions. Immunohaematological studies were performed using a gel-based technology. At admission, the patient had a low RBC count and an increased mean corpuscular volume with normal haemoglobin. A double population appeared in the RBC histogram. However, the peripheral blood smear was normal and macrocytosis was absent when heparin or citrate was used instead of EDTA. Later studies revealed that the patient's serum was able to induce macrocytosis of control RBC only in the presence of EDTA. An EDTA-dependent panagglutinin was then indentified that produced mixed field agglutination. These findings provide evidence of a haemagglutination induced by EDTA as a source of pseudomacrocytosis.

  • Hematopathology
  • Anticoagulants
  • Analytical Methods

Statistics from

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.