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CD10 is a cell surface neutral endopeptidase expressed by lymphoid precursor cells, germinal centre B lymphocytes, and some myelocytes.1 Therefore, it is used as marker for the categorisation of acute leukaemia and subclassification of lymphomas.2 With focus on germ cell tumours Del Sordo et al3 reported in a cohort of 135 germ cell tumours expression of 92% for seminomas, whereas non-seminomatous germ cell tumours were negative. The authors concluded that CD10 serves to differentiate seminoma from non-seminomatous germ cell tumours. The aim of this study was to describe CD10 expression in the so far largest germ cell tumour set and to validate the findings of previous studies.
Materials and methods
The tissue microarray used for this study has previously been described.4 It includes 325 testicular germ cell tumours: 207 classic seminomas, 4 spermatocytic seminomas, 19 pure embryonal carcinomas, 1 pure mature teratoma and 94 mixed germ cell tumours. In mixed germ cell tumours each component was separately punched and represented on the tissue microarray. Finally, 442 different histologic tumour components were considered for evaluation.
CD10 was detected using the prediluted antibody from Ventana (790–4506). The staining was performed with the Ventana Benchmark automated staining system (Ventana Medical Systems, Tucson, AZ). Samples were dichotomised into positive versus negative. The intensity of immunostaining was graded as absent, weak, moderate and strong. The threshold for …
Contributors Author contributions: PKB had full access to all the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis. Study concept and design: CDF, TS, QZ, AB, HM, PKB. Acquisition of data: CDF, PKB. Analysis and interpretation of data: CDF, PKB. Drafting of the manuscript: CDF, TS, QZ, AB, HM, PKB. Critical revision of the manuscript for important intellectual content: CDF, TS, QZ, AB, HM, PKB. Supervision: PKB.
Competing interests None.
Ethics approval Cantonal ethics committee Zurich.
Provenance and peer review Not commissioned; internally peer reviewed.