Statistics from Altmetric.com
Circulating tumour cells (CTCs) attract a significant interest both in basic cancer research and in clinical oncology as a biomarker for diagnosis and prognosis of cancer. A number of techniques have been developed to trap and visualise CTCs.1
We postulated that antibody-independent approaches for collection of CTCs have a number of advantages, although subsequent effective visualisation of CTCs to distinguish them from surrounding white blood cells or non-cancerous epithelial cells is critical for their applicability for use in standard clinical settings.2 ,3 One potential solution is the usage of sensitive staining techniques such as recently developed ViewRNA in situ hybridisation by Affymetrix/Panomics. This approach is based on the use of specifically designed oligonucleotide probes against mRNA of genes of interest. These probes are hybridised to mRNA in fixed cells followed by binding of branched nucleic acids and fluorescent or chromogenic staining, which allow simultaneous identification of two or three targets, …
If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.