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HBME-1 is expressed by erythroid precursors in early maturation stage and can be a valuable tool for evaluation of dyserythropoiesis in bone marrow core biopsy specimens
  1. Riad Arana1,
  2. Daniel Lusina2,
  3. Thorsten Braun3,
  4. Rémi Letestu2,4,
  5. Claude Gardin3,4,
  6. Antoine Martin1,4
  1. 1Department of Pathology, Avicenne Hospital, Bobigny, France
  2. 2Department of Biological Haematology, Avicenne Hospital, Bobigny, France
  3. 3Department of Clinical Haematology, Avicenne Hospital, Bobigny, France
  4. 4University of Paris 13, Bobigny, France
  1. Correspondence to Dr Riad Arana, Department of Pathology, Avicenne Hospital, 125 Rue de Stalingrad, Bobigny 93000, France; ryiad83{at}


The reaction of Hector Battifora mesothelial epitope-1 (HBME-1) antibody with scattered pronormoblasts in normal bone marrow core biopsy specimens has been reported. This study evaluated the immunohistochemical profile of HBME-1 in a panel of 52 normal, dyserythropoietic and neoplastic marrow samples. We compared the staining property of HBME-1 with that of the commonly used erythroid marker, glycophorin A (CD235a) and in each case, we semi-quantitatively evaluated the HBME-1/CD235a-positive cells ratio. In normal samples, HBME-1 labelled scattered immature erythroid precursors. In dyserythropoietic specimens, HBME-1 stained nucleated erythroid precursors in varying degrees, from pronormoblast through normoblast stages, with the highest intensity in immature forms. Overall, the cellular background of non-erythroid progenitors, erythrocytes and neoplastic cells did not react with HBME-1, except in leukaemia cases with myelodysplasia-related changes. Our study shows that HBME-1 is a useful marker to identify immature erythroid precursors and that an HBME-1/CD235a-positive cells ratio ≥10% is associated with dyserythropoiesis.


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