Article Text

Mechanisms and therapeutic effectiveness of lactobacilli
  1. Alessandro Di Cerbo1,
  2. Beniamino Palmieri2,
  3. Maria Aponte3,
  4. Julio Cesar Morales-Medina4,
  5. Tommaso Iannitti5
  1. 1School of Specialization in Clinical Biochemistry, “G. d'Annunzio” University, Chieti, Italy
  2. 2Department of General Surgery and Surgical Specialties, University of Modena and Reggio Emilia Medical School, Surgical Clinic, Modena, Italy
  3. 3Department of Agriculture, University of Naples “Federico II”, Portici, Naples, Italy
  4. 4Centro de Investigación en Reproducción Animal, CINVESTAV- Universidad Autónoma de Tlaxcala, Tlaxcala, México
  5. 5Department of Neuroscience, Sheffield Institute for Translational Neuroscience (SITraN), University of Sheffield, Sheffield, UK
  1. Correspondence to Dr Tommaso Iannitti, Sheffield Institute for Translational Neuroscience, University of Sheffield, 385A Glossop Road, Sheffield, S10 2HQ, UK; tommaso.iannitti{at}


The gut microbiome is not a silent ecosystem but exerts several physiological and immunological functions. For many decades, lactobacilli have been used as an effective therapy for treatment of several pathological conditions displaying an overall positive safety profile. This review summarises the mechanisms and clinical evidence supporting therapeutic efficacy of lactobacilli. We searched Pubmed/Medline using the keyword ‘Lactobacillus’. Selected papers from 1950 to 2015 were chosen on the basis of their content. Relevant clinical and experimental articles using lactobacilli as therapeutic agents have been included. Applications of lactobacilli include kidney support for renal insufficiency, pancreas health, management of metabolic imbalance, and cancer treatment and prevention. In vitro and in vivo investigations have shown that prolonged lactobacilli administration induces qualitative and quantitative modifications in the human gastrointestinal microbial ecosystem with encouraging perspectives in counteracting pathology-associated physiological and immunological changes. Few studies have highlighted the risk of translocation with subsequent sepsis and bacteraemia following probiotic administration but there is still a lack of investigations on the dose effect of these compounds. Great care is thus required in the choice of the proper Lactobacillus species, their genetic stability and the translocation risk, mainly related to inflammatory disease-induced gut mucosa enhanced permeability. Finally, we need to determine the adequate amount of bacteria to be delivered in order to achieve the best clinical efficacy decreasing the risk of side effects.


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The impact of the gastrointestinal (GI) tract on brain functions and behaviour including anxiety, mood, cognition and pain regulation has been recognised since the 19th century as Hipocrates’ dictum stated “Let the food be thy medicine and medicine be thy food”.1 Therefore, the gut-brain axis has been proposed as a homoeostatic route of communication using neuronal, hormonal and immunological pathways.1–3 The GI tract, which is an active part of this axis, is harboured by approximately 100 trillion organisms, mainly anaerobes, which constitute the microbiome and exceed 10 times the overall number of cells present in the human body.4 ,5 The microbiome plays a key role in the development and functionality of the innate and adaptive immune responses.1 Among microbiome-composing organisms, lactobacilli can inhibit the growth of pathogenic bacteria and have a favourable safety profile.6 However, different species of the genus Lactobacillus (L.) can produce different particular responses in the host, and the effects exerted by some strains of the same species may not be beneficial.7

Aim and searching criteria

In this review, we summarise the experimental and clinical evidence on lactobacilli by providing a comprehensive overview of their efficacy for treatment of numerous pathologies and outlining new therapeutic trends. We searched Pubmed/Medline using the keyword ‘Lactobacillus’. Selected papers from 1950 to 2015 were chosen on the basis of their content. Relevant clinical and experimental articles that used lactobacilli as therapeutic agents and written in English language have been included. Clinical findings organised by pathology are summarised in tables 115.

Table 1

Lactobacilli displaying ability to adhere to the gastrointestinal tract mucosa

Table 2

Clinical studies showing efficacy of lactobacilli for treatment of cancer

Table 3

Clinical studies of lactobacilli showing efficacy for treatment of vaginal disorders

Table 4

Clinical studies of lactobacilli showing efficacy for treatment of hypercholesterolaemia

Table 5

Clinical studies of lactobacilli showing inhibitory activity against H. pylori infection

Table 6

Clinical studies of lactobacilli showing efficacy for treatment of oxaluria

Table 7

Clinical studies of lactobacilli showing efficacy for treatment of mastitis

Table 8

Clinical studies of lactobacilli showing immunomodulatory activity in various pathologies

Table 9

Clinical studies of lactobacilli showing efficacy for treatment of gastrointestinal pathologies

Table 10

Clinical studies of lactobacilli showing ability to survive in the gastrointestinal tract

Table 11

Clinical studies of lactobacilli showing efficacy for treatment of diarrhoea

Table 12

Clinical studies of lactobacilli showing efficacy for treatment of periodontal disease

Table 13

Clinical studies of lactobacilli showing efficacy for treatment of type-2 diabetes

Table 14

Clinical studies of lactobacilli showing efficacy for treatment of various pathologies

Table 15

Clinical studies reporting side effects associated with Lactobacillus therapy

Experimental evidence

Adhesion to the gastrointestinal mucosa

Dietary changes, antibiotic exposure and infections may cause dysbiosis, a perturbation of the microbiome-host symbiosis that favours the invasion and growth of pathogenic species to the detriment of health-promoting bacteria, including lactobacilli, within the GI tract.8 ,9 Indeed, adhesion of lactobacilli to the host's GI tract, by means of an interaction with toll-like receptors, is of crucial importance due to its ability to trigger the host's immune response.10 ,11 Nevertheless, adhesion to the GI tract can also be driven by surface proteins and fatty acids, as observed for L. rhamnosus PEN,12 and proteinaceous surface layer components, as observed for L. plantarum 91.13 Therefore, the ability of lactobacilli to adhere and colonise the GI tract mucosa has been investigated in the clinical setting and is summarised in table 1.14–17

Antitumour activity

Intestinal bacteria produce mutagens such as deoxycholic acid from primary bile acids or by enzymatic conversion when foreign compounds, such as nitroaromatics, azo compounds and nitrates, are ingested.18 Lactobacilli are capable of competitively inhibiting carcinogen and mutagen formation, altering overall metabolism, adsorbing and removing toxic and mutagenic metabolites and producing protective metabolites.19 In the context of colorectal cancer, the prevention mechanism exerted by probiotics may be a combination of different actions such as intestinal microbiota modification,20–26 inactivation of cancerogenic compounds,27–35 competition with putrefactive and pathogenic microbiota,36–40 improvement of the host's immune response,41–55 enhancement of natural killer cell cytotoxicity56 and inhibition of interleukin (IL) 6 production in the colonic mucosa57 counteracting cancer development by antiproliferative effects58 via regulation of apoptosis and cell differentiation,59–67 fermentation of undigested food68–73 and inhibition of tyrosine kinase signalling pathways.74 Experimental studies have also shown that lactobacilli contained in dietary supplements and fermented food, such as yogurt heat-killed L. casei strain Shirota (LC 9018)54 reduce colon cancer risk.75–77 These activities have been ascribed to the alteration of gut microbiota and, subsequently, to the inhibition or the induction of colonic enzymes controlling the growth of harmful bacteria, improving immune function and stimulating the production of metabolites possessing antitumour activity. Clinical studies showing efficacy of lactobacilli for treatment of cancer have been summarised in table 2.

Antitoxic activity

Lactobacilli display detoxifying properties and their ability to neutralise toxins81 or toxic compounds82 is important to maintain the host's health. For instance, L. reuteri CRL 1098 and L. acidophilus CRL 1014 showed the ability to enhance tumour necrosis factor (TNF)-α response to ochratoxin A, a widespread mycotoxin from Aspergillus and Penicillium species. This mycotoxin can contaminate food products83 and induce hepatotoxicity, nephrotoxicity and immunotoxicity,84 thus increasing TNF-α production and diminishing toxin-induced apoptosis.83 Individual treatment with L. plantarum 2 017 405, L. fermentum 353, L. acidophilus DSM 21007 and L. rhamnosus GG antagonised C. difficile isolated from faecal specimens from adult patients affected by diarrhoea, as observed by measurement of the inhibition zone.85 Another L. strain, L. reuteri RC-14,86 produced small signalling molecules able to interfere with a key regulator of virulence genes, agr. Additionally, L. reuteri RC-14 repressed the expression of toxic shock syndrome toxin-1 in menstrual toxic shock syndrome induced by Staphylococcus (S.) aureus strains. Quantitative real-time polymerase chain reaction (PCR) data revealed that transcription from the toxic shock tst promoter was strongly inhibited in culture supernatant in presence of L. reuteri RC-14. Moreover, a transcriptional level alteration of virulence-associated regulators was observed, providing a unique mechanism by which endogenous or exogenous lactobacilli can attenuate production of virulence factors. This study highlighted the existence of a crosstalk mechanism between two distinct bacterial signalling systems, alteration in the transcriptional levels of virulence-associated regulators sarA and saeRS and transcription inhibition from Ptst, P2 and P3 promoters, providing a potential defensive mechanism against S. aureus infections. Therefore, administration of well-characterised lactobacilli can be helpful to overcome antibiotic-related complications, such as antibiotic resistance. Based on 16SrDNA sequences and non-coding fragments characterisation of different lactobacilli, Fei and coworkers reported a significantly high nitrite degradation capacity exerted by L. sp DMDL 9010 after a 24 h fermentation in the medium.87 Compound degradation activity of lactobacilli has also been observed for cadmium after high dietary exposure.88 In this regard, two L. kefir strains, CIDCA 8348 and JCM 5818, can remove cadmium cations when cocultured with a human hepatoma cell line, HepG2.89 Particularly, L. kefir JCM 5818 is more efficient in protecting cells from cadmium toxicity. Therefore, since consumption of harmful metals is a growing medical issue, the regular administration of formulations containing the above mentioned strains might be useful to prevent toxin compound-induced lipid peroxidation and free radical production.

Vaginal colonisation

Vaginal microbiota is dominated by lactobacilli.90 When the balance among bacterial species within this environment is altered, antibacterial defense mechanisms lose their efficacy leading to pathogenic bacteria proliferation.90 For instance, reduction in the number of vaginal lactobacilli and their antimicrobial properties (such as lysostaphin expression in order to cleave the cell wall of S. aureus thus inhibiting its growth),91 and H2O2 production,92 cause bacterial vaginosis, the most common symptomatic microbial imbalance.93 In patients affected by bacterial vaginosis, lactobacilli are replaced by Gardnerella vaginalis,92 ,94 Candida (C.) albicans,95 S. aureus,91 ,96 Neisseria gonorrhoeae40 or other anaerobic bacteria. Uncontrolled growth of anaerobic bacteria such as C. albicans and subsequent vaginal colonisation may lead to vulvovaginal candidiasis,97 which is estimated to occur at least once during the lifetime of 75% of the female population.98 Vaginal microbial imbalance may also represent an important risk factor for increased risk of urinary tract infections and pregnancy complications, such as endometritis, chorioamnionitis, preterm birth and intrauterine death.99 Intravaginal colonisation by bacterial strains with high haemolytic activity and pigment production [eg, group B streptococci (GBS)] is one of the most important risk factors for disease development in newborns.100 Therefore, a murine model was proposed to determine if L. reuteri CRL1324 would exert a preventive effect on vaginal colonisation by Streptococcus (St.) agalactiae NH17.100 Following L. reuteri CRL1324 administration, a reduced leucocyte influx induced by St. agalactiae NH17 and a preventive effect on its vaginal colonisation were observed prior to the GBS challenge. Although GBS colonization occurs in up to 50–70% of neonates born from colonized mothers,101 the introduction of new antimicrobial agents, such as L. reuteri CRL1324, could be considered a valuable and safer alternative to antibiotics to reduce infections caused by GBS. Clinical studies of lactobacilli showing efficacy for treatment of vaginal disorders have been summarised in table 3.

Cholesterol-lowering activity

There is an increasing demand for non-pharmacological therapies to improve cholesterol profile due to the cost and side effects associated with available pharmacological treatments for cholesterol-related diseases. Hence great attention has been given to lactobacilli due to their effectiveness in modulating lipid metabolism reducing statin requirement (statins inhibit the enzyme 3-hydroxy-3-methylglutaryl-coenzyme A reductase that produces about 70% of the total body cholesterol)110 ,111 and serum cholesterol level by means of bile salt hydrolase that has a direct impact on the host's bile salt metabolism accounting for the formation of deconjugated bile acids.112 Furthermore, cholesterol-reducing properties were also observed for L. oris HMI118, HMI28, HMI43, HMI68 and HMI74 isolated from breast milk.113 Although all the tested strains assimilated cholesterol even in the absence of bile salts, surviving in the acidic conditions of the intestine and tolerating high bile concentrations, L. oris HMI68 showed the highest cholesterol assimilation deconjugating sodium glycocholate (the most predominant bile salt in the human intestine) and sodium taurocholate. Cholesterol assimilation has also been evaluated as a possible therapeutic approach to reduce the risk for cardiovascular diseases.114 In this regard, Tomaro-Duchesneau and coworkers investigated the ability of 11 L. strains (L. reuteri NCIMB 11951, 701359, 702655, 701089 and 702656, L. fermentum NCIMB 5221, 8829, 2797, L. rhamnosus ATCC 53103 GG, L. acidophilus ATCC 314 and L. plantarum ATCC 14917) to assimilate cholesterol. While L. plantarum ATCC 14917 was the best cholesterol assimilator in de Man, Rogosa and Sharpe broth, L. reuteri NCIMB 701089 assimilated over 67% of cholesterol under physiological intestinal conditions. The hypocholesterolaemic effect of all strains, particularly of L. reuteri NCIMB 701089, was linked to intrinsic bile salt hydrolase activity, assimilation and incorporation in cellular membranes and compound production, for example, ferulic acid,115 able to inhibit the activity of enzymes, including 3-hydroxy-3-methylglutaryl-coenzyme A reductase.116 More recently, cholesterol-reducing L. spp. GI6, GI9, GI11 and GI15 were also isolated from traditionally fermented south Indian koozh and gherkin (a variety of cucumber).117 L. GI9 was able to survive at pH 2.0 and 0.50% bile salt for 3 h without losing its viability also exhibiting the maximum cholesterol reduction. Nevertheless, all tested lactobacilli exhibited inhibitory activity against several pathogens including Escherichia coli MTCC 1089, Pseudomonas (P.) aeruginosa MTCC 2642, S. aureus MTCC 7443, Klebsiella (K.) pneumoniae MTCC 7028, Bacillus subtilis MTCC 8561 and C. albicans BS3 and were able to deconjugate bile salts. Clinical studies of lactobacilli showing efficacy for treatment of hypercholesterolaemia have been summarised in table 4.

Antioxidant activity

Lactobacilli can prevent lipid peroxidation122 and free oxygen radical production123 due to their ability to create the low oxidation-reduction potential required for their optimal growth.124 Amaretti and coworkers combined the strains Bifidobacterium (B.) animalis subsp lactis DSMZ 23032, L. acidophilus DSMZ 23033 and L. brevis DSMZ 23034 and administered them for 18 days to rats previously treated with doxorubicin, an anthracycline antibiotic.125 Analysis of plasma antioxidant activity, glutathione concentration, as well as levels of reactive oxygen species, revealed a reduction in doxorubicin-induced oxidative stress, thus supporting antioxidant activity of these probiotics.

Antibacterial and antiviral activity

Probiotic strains beneficially affect the host by replacing pathogenic bacteria in the GI tract and modulating immune responses.126 Experimental studies have shown that lactobacilli, which can adhere to enterocytes, are effective in preventing the enteropathogen-mediated infection by competing for nutrients127 and binding sites (eg,inducing intestinal mucin gene expression),128–132 by secreting antimicrobial substances133 such as organic acids,134–142 bacteriocins143–145 and hydrogen peroxide146–152 and eventually by counteracting the spread within the colonised body,153–155 reducing gut pH133 ,141 ,156 and producing biosurfactants.157–159 As far as bacterial activity is concerned, L. plantarum GK81, L. acidophilus GK20 and L. plantarum JSA22 inhibit Salmonella spp infection in intestinal epithelial cells160 ,161 and L. acidophilus strain inhibits various pathogenic bacteria including P. aeruginosa, E. coli, Enterobacter and K. spp.150 With reference to antiviral activity, lactobacilli harbour surface layer proteins involved in the enhancement of viral entry.162 Moreover, increasing data indicate that abnormal vaginal flora lacking lactobacilli can facilitate viral sexually transmitted disease diffusion such as in the case of HIV,163 human papilloma virus164 and herpes simplex virus 2.165 In this context, lactobacilli can exert an important role protecting the vaginal environment and reducing the risk of virus transmission.

Helicobacter pylori infection

Helicobacter (H.) pylori, a gram-negative microaerophilic human gastric pathogen, is the main cause of chronic gastritis, gastric cancer and peptic ulcer disease.166 Antibiotic treatment for H. pylori infection is associated with serious side effects and therefore there is an increasing demand for new treatments. Lactobacilli167 ,168 have been extensively investigated for treatment of H. pylori infections. Numerous L. strains, that is, L. gasseri Chen, L. plantarum 18,167 L. gasseri OLL2716,168 L. reuteri,169 L. rhamnosus GG, L. rhamnosus Lc705, Propionibacterium (P.) freudenreichii subsp shermanii Js,170 L. delbrueckii subsp bulgaricus 48, 144 and GB,171 L. rhamnosus LC705, P. freudenreichii ssp shermanii JS,168 L. acidophilus LB,172 L. plantarum MLBPL1, L. rhamnosus GG and L. lactis137 possess a neutralising activity against H. pylori. The same activity was also observed for heat-killed L. johnsonii Lal and L. helveticus173 as well as for L. gasseri OLL2716,174 as measured by 13C-urea breath test. The suppressive effect of lactobacilli on H. pylori infection in vivo and in vitro has been reviewed.175–177 For instance, L. johnsonii 1088 suppressed gastric acid secretion in mice via decreasing the number of gastrin-positive cells in the stomach.176 Therefore L. johnsonii 1088 can be considered a valid add-on therapy to the gold standard treatment for H. pylori eradication consisting of a proton pump inhibitor (PPI), amoxicillin and clarithromycin, and can also be used for prophylaxis of gastroesophageal reflux disease that can develop following H. pylori eradication. Nevertheless, the use of a PPI can also modify the gut microbiota causing dysbiosis.178–180 In this regard, adding L. paracasei subsp paracasei F19 to triple therapy is a promising combination to counteract the effects of PPIs on intestinal dysbiosis.181 Clinical studies of lactobacilli showing inhibitory activity against H. pylori infection have been summarised in table 5.

Kidney disease

The last stage of chronic kidney disease induces an increase in plasma concentration of uraemic wastes and requires kidney transplantation or chronic dialysis.190 Many studies support the probiotic approach as an alternative therapy for management of end-stage renal disease191 and to relieve the ‘uraemic’ condition.189 ,192–194 In particular, a high urease activity was observed for S. spp, L. casei, K. aerogenes and Enterococcus faecium in the sheep rumen.192 At the same time, the ability to degrade biogenic amines (BAs) was also assessed by Capozzi and coworkers.193 They isolated two lactobacilli (L. plantarum NDT 09 and L. plantarum NDT 16) from wine and found that they were able to degrade tyramine (22.12%) and putrescine (31.09%), respectively. L. casei 4a and 5b, isolated from Zamorano cheese, also inhibited tyramine along with histamine, another BA.194 However, BA degradation is not the only mechanism under investigation for treatment of end-stage renal disease and uraemic condition. The ability to degrade oxalate and to survive within the GI tract of a range of B. and L. species, isolated from the canine and feline GI tract, has also been evaluated. In vitro oxalate degradation was detected for 11 out of 18 L. strains (8 L. animalis and 3 L. murinus), but not for any of the B. strains.195 Rats were fed on four selected strains (L. animalis 223C, L. murinus 1222, L. animalis 5323 and L. murinus 3133) for 4 weeks; urinary oxalate levels were significantly reduced only in those rats fed on L. animalis 5323 and L. animalis 223C. Oxalate-degrading activity has also been assessed for other lactobacilli.196 L. paracasei LPC09 displayed the highest oxalate-degrading activity converting 68.5% of ammonium oxalate followed by L. gasseri LGS01 (68.4%), L. gasseri LGS02 (66.2%), L. acidophilus LA07 (54.2%) and L. acidophilus LA02 (51.3%). The use of lactobacilli as agents able to integrate into the host's gut microbiota may thus be considered helpful in reducing oxaluria and preventing or decreasing the incidence and severity of kidney stone formation. Clinical studies of lactobacilli showing efficacy for treatment of oxaluria have been summarised in table 6.


Mastitis is an infectious inflammation of one or more breast lobules198 with S. aureus and S. epidermidis being the most frequent aetiological agents199 and with a prevalence of 3–33% among breastfeeding mothers.200 Multidrug resistance and biofilm formation by pathogenic bacteria account for the lack of efficacy of antibiotics used for treatment of mastitis.201 In this context, new strategies based on probiotics, as alternatives or complements to antibiotic therapy for the management of mastitis, are gaining a prominent role. Clinical studies of lactobacilli showing efficacy for treatment of mastitis have been summarised in table 7.

Immunomodulatory activity

Lactobacilli are potential adjuvants triggering mucosal and systemic immune responses.204 The immunomodulatory effects of lactobacilli observed in various physiological systems include increased natural killer cell cytotoxicity205 ,206 and induction of interferon-γ production205–213 and cytokine expression.205–210 ,212–216 In order to exert these immunomodulatory effects, lactobacilli must resist to digestive system processes217 and adhere to the host's intestinal epithelium.218 Lactobacilli (in particular L. acidophilus) can also be administered together with bifidobacteria in order to enhance the immune system.219 ,220 This effect is accomplished by enhancing systemic/local immunity221 and concurrently attenuating systemic stress response.222 Clinical studies of lactobacilli showing immunomodulatory activity in various pathologies have been summarised in table 8.

Gastrointestinal pathologies

Even if the pathogenesis of irritable bowel syndrome (IBD) remains unknown, the luminal microbiome plays a key role in triggering and maintaining a balanced environment within the GI tract.226 Dysbiosis may also play a key role in IBD.227 Evidence from animal models228 and clinical observations229 outlined the putative therapeutic role of probiotic strains for IBD treatment. Restoring microbiota-host symbiosis can represent a promising approach for treatment of the above mentioned conditions and can be applied to other GI pathologies, as summarised in table 9.

Gastrointestinal tract survival

Strains belonging to L. and B. genera are the most studied in clinical practice.234 The number of bacterial strains that reach the gut mucosa and colon, depends on several factors such as strain used, gastric transit survival,15 ,235 and acid and bile tolerance.236 Clinical studies of lactobacilli showing ability to survive in the GI tract have been summarised in table 10.


Imbalance in the gut flora can cause diarrhoea, enteritis and colitis, among other diseases. VSL#3 (St. thermophilus, B. breve, B. longum, B. infantis, L. acidophilus, L. plantarum, L. casei and L. bulgaricus) and L. casei DN-114 001 administration decreased the incidence and frequency of radiation therapy-induced diarrhoea.242 Diarrhoea is also frequent during antibiotic therapy causing gut flora imbalance.243 ,244 Clostridium (C.) difficile infection, a gram positive, spore-forming anaerobe, can cause antibiotic-associated diarrhoea and colitis in humans.245 ,246 Boonma and coworkers investigated the probiotic effect of L. rhamnosus L34 and L. casei L39, two vancomycin-resistant lactobacilli, on the suppression of IL-8 production in response to C. difficile infection.247 While L. casei L39 suppressed the activation of phosphonuclear factor κ-light-chain-enhancer of activated B cells and phospho-c-Jun in HT-29 cells, L. rhamnosus L34 and L. casei L39 decreased the production of C. difficile-induced granulocyte-macrophage colony-stimulating factor. Moreover, L. acidophilus GP1B cell extract decreased transcriptional levels of luxS, tcdA, tcdB and txeR genes of C. difficile, thus reducing virulence in vitro.248 In vivo, survival rates at 5 days for mice that received C. difficile and L. acidophilus GP1B cell extract or L. acidophilus GP1B were reduced up to 80%. Therefore, in vitro and in vivo investigations have showed that lactobacilli presented antibacterial effects. Clinical studies of lactobacilli showing efficacy for treatment of diarrhoea have been summarised in table 11.

Periodontal disease

Periodontal diseases can be divided into gingivitis and periodontitis.264 While the first condition is characterised by inflammation of the gingiva,265 the second is a progressive destructive disease which involves tooth supporting tissues such as the alveolar bone.266 Periodontitis is mainly characterised by the presence of Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia and Aggregatibacter actinomycetemcomitans which colonise the subgingival sites escaping the host defense system and eventually causing tissue damage.267 Among antimicrobial and bacteriostatic agents, chlorhexidine is the gold standard for treatment of periodontitis because of its broad-spectrum antibacterial activity.268–270 However, a number of side effects, such as brown teeth discolouration, salt taste perturbation, oral mucosal erosions and enhanced supragingival calculus formation, have been reported and they have limited chlorhexidine long-term use.271 Evidence has shown the effectiveness of lactobacilli in reducing gingival inflammation and the number of cariogenic periodontopathogenic bacteria.272 Further studies have shown that lactobacilli reduced the prevalence of moderate-to-severe gingival inflammation and improved plaque index (clinically used to measure the state of oral hygiene)273 ,274 as well as decreased the levels of the proinflammatory cytokines TNF-α, IL-8 and IL-1β.275 Saha and coworkers investigated the role of selected lactobacilli in St. mutans inhibition.276 L. reuteri strains NCIMB 701359, NCIMB 701089, NCIMB 702655 and NCIMB 702656 inhibited St. mutans to non-detectable levels (<10 CFU/mL) suggesting their use as therapeutic agents for caries and periodontal disease. Moreover, L. fermentum NCIMB 5221 inhibited St. mutans buffering the pH (4.18) of saliva containing this pathogenic microbe and coaggregating with it also showing high levels of sucrose consumption. Altogether, these studies suggest that lactobacilli may improve oral health and reduce periodontopathogenic bacteria. Clinical studies of lactobacilli showing efficacy for treatment of periodontal diseases have been summarised in table 12.


Diabetes, a chronic metabolic disease, is characterised by elevated blood glucose levels due to either insufficient insulin production by β-islet cells (type-1 diabetes) of the pancreas or impaired insulin sensitivity of insulin target organs, that is, adipose tissue, liver and muscle (type-2 diabetes or diabetes mellitus).277 In this context, inflammatory immune responses play a crucial role in the progression of both types of disease.278–280 As for type-2 diabetes, it is generally treated with intestinal α-glucosidase inhibitors.281 In this regard, Actinoplanes spp have been shown to naturally produce potent α-glucosidase inhibitor compounds including acarbose. Panwar and coworkers first isolated and extracted lactobacilli from human infant faecal samples and evaluated their inhibitory activity against intestinal maltase, sucrose, lactase and amylase, all enzymes involved in hydrolysis of carbohydrates.281 This study showed that several strains exert powerful inhibitory effects against the aforementioned enzymes and L. rhamnosus reduced glucose excursions in rats during a carbohydrate challenge by inhibiting β-glucosidase as well as α-glucosidase activities. Even if further studies are certainly needed, administration of lactobacilli may represent a promising novel therapeutic tool for treatment of diabetes. Clinical studies of lactobacilli showing efficacy for treatment of diabetes have been summarised in table 13.


Osteoarthritis, a chronic joint disease characterised by progressive cartilage degeneration, subchondral bone sclerosis, synovial inflammation and osteophyte formation,283 mainly affects weight-bearing joints such as knees and hips. A chronic inflammatory response occurs in synovial membranes with increased expression of proinflammatory cytokines and mononuclear cell infiltration.284 Oral intake of skimmed milk fermented with L. delbrueckii subsp bulgaricus OLL1073R-1 inhibits the development of collagen-induced arthritis in mice. Moreover, a reduced secretion of IFN-γ was also observed in these animals.285 Moreover, L. casei suppresses experimental rheumatoid arthritis by downregulating Th1-type inflammatory responses286 and its coadministration with type-II collagen and glucosamine decreased the expression of various proinflammatory cytokines and matrix metalloproteinases, upregulating anti-inflammatory cytokines.287 The immunomodulating activity of lactobacilli in rheumatoid arthritis was also confirmed by a trial on 45 adult men and women affected by this pathology.288 Bacillus coagulans GBI-30, 6086, administered for 60 days in addition to standard antiarthritic medications, resulted in an improvement in the Patient Pain Assessment score and statistically significant improvement in Pain Scale with respect to placebo.

Other pathologies

Lactobacilli have found application for treatment of several other pathologies. For instance, L. plantarum strain K21 that inhibits lipid accumulation in 3T3-L1 preadipocytes, alleviated body weight gain and epididymal fat mass accumulation, reduced plasma leptin levels, decreased cholesterol and triglyceride levels as well as mitigated liver damage in a mouse model of diet-induced obesity.289 Antilipidemic effects of lactobacilli were also evaluated along with memory-enhancing activity in aged Fischer 344 rats.290 A probiotic mixture of L. plantarum KY1032 and L. curvatus HY7601 was provided once a day for 8 weeks. A significant inhibition of age-dependent increase in blood triglycerides and a reduction in high-density lipoprotein cholesterol was observed. Moreover, the mixture restored age-reduced spontaneous alternation in the Y-maze task and age-suppressed doublecortin and brain derived neurotrophic factor expression. In addition, suppression of p16, p53 and cyclooxygenase-2 expression, phosphorylation of protein kinase B and mammalian target of rapamycin and activation of nuclear factor κ-light-chain-enhancer of activated B cells were observed, thus suggesting a therapeutic role of such mixture in ameliorating age-dependent memory deficit and lipidemia in aged subjects. Clinical studies of lactobacilli showing efficacy for treatment of various pathologies have been summarised in table 14.

Side effects of lactobacilli

The widespread clinical use of lactobacilli, even for pathologies that are challenging to treat, has highlighted potential translocations or mutations and untoward effects such as sepsis,296–301 endocarditis,302–305 bacteraemia299 ,306–319 and even death.320 Evidence regarding lactobacilli side effect profile has been summarised in table 15.


The mammalian gut microbiome interacts with several physiological systems within the host contributing to multiple biological processes. In vitro and in vivo investigations have shown that prolonged probiotic administration induces qualitative and quantitative modifications in complex, well-settled microbial ecosystems through bacteriocin substrate competition and possibly other mechanisms that still need to be acknowledged. Probiotics can modulate the GI tract microbial ecology exerting immunomodulatory effects that are therapeutic at least for treatment of specific pathologies.331 Our review takes into account the available clinical and experimental evidence on the use of lactobacilli in order to give an overview of their suitability to be enclosed in well defined updated therapeutic protocols for specific pathologies. A limited number of studies have already tested the hypothesis that lactobacilli could be combined with bifidobacteria or other nutrients, such as fibres, in order to enhance the bioavailability, mucosal adhesion and therapeutic effectiveness of lactobacilli. Further studies are certainly warranted to determine the most effective combinations for treatment of individual pathologies. The claim that pools of lactobacilli could better survive within the gut lumen and even in the colon, and stably integrate within the pre-existing microbiome, has never been proved in terms of dose-effect and risk of sepsis and bacteraemia. We do not have enough information about the long-term genetic stability (with some exceptions such as L. paracasei subsp paracasei F19332 ,333), the antibiotic susceptibility and translocation rate of L. strains.334–336 Therefore, further investigations are required to fill in this gap. We would also like to point out the increasing interest in lactobacilli used for industrial food fermentation which has reached a high degree of sophistication that could be useful also for medical applications.337 For example, various novel biological modifications have been introduced such as the lysostaphin-expressing gene to prevent growth of toxic shock syndrome toxin 1 producing strains of S. aureus.338

However, since data concerning the safety and genetic stability of lactobacilli is still limited, toxicological studies evaluating the effects of their genetic modification on the homeostasis of the host organism are still required. Ongoing research on the human microbiome composition will likely yield new species of the genus L. that might also have therapeutic applications for specific pathologies.

Take home messages

  • Experimental and clinical evidence supports effectiveness of lactobacilli for treatment of several pathological conditions.

  • Long-term consumption of lactobacilli induces qualitative and quantitative modifications in the human gastrointestinal microbial ecosystem.

  • Pharmacological profile of lactobacilli needs to be further characterised in order to avoid translocation-related risks.


JCMM acknowledges CONACyT for membership.


Supplementary materials

  • Abstract in Italian

    This web only file has been produced by the BMJ Publishing Group from an electronic file supplied by the author(s) and has not been edited for content.


  • Correction notice Since this paper was published online the author has changed the formatting of tables 2–15, corrected the units in these tables and added italics to gene names throughout the paper.

  • Handling editor Slade Jensen

  • Contributors All the authors contributed equally to this work.

  • Competing interests None declared

  • Provenance and peer review Not commissioned; externally peer reviewed.