Article Text
Abstract
Heparin can induce the formation of antibodies against a heparin complex with a platelet factor 4 (PF4), leading to platelet activation and the development of heparin-induced thrombocytopaenia (HIT). Because screening ELISA does not discriminate between platelet activating and non-activating anti-heparin/PF4 antibodies, each positive result is confirmed by an additional functional assay. We analysed 1004 sera of patients with suspected HIT. Optical density (OD) values of ELISA-positive results were correlated with the risk for a positive result with our functional flow cytometric assay. Only 10.7% were ELISA positive and 59.8% of those were positive with the functional assay. The positive functional assay was found in 23.4% of patients with OD<1.0, in 57.7% with 1.0<OD<2.0 and in 94.1% with OD>2.0. Although our results showed that higher ELISA OD values increasethe possibility of the presence of platelet-activating anti-heparin/PF4 antibodies , there is no need for improving ELISA cut-off value for positive result.
- heparin-induced thrombocytopenia
- HIT
- OD value
- functional assay
- platelet activation
- flow cytometry
Statistics from Altmetric.com
Footnotes
Handling editor Mary Frances McMullin
Contributors EM: conception and design, collection and assembly of data, data analysis and interpretation, manuscript writing. MMK: performing the literature search, data analysis and interpretation. PK: collection and assembly of data, data analysis and interpretation. PR: final approval of the manuscript.
Competing interests None declared.
Request Permissions
If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.