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Human polyomavirus DNA detection in keratoacanthoma and Spitz naevus: no evidence for a causal role
  1. Linnea Haeggblom,
  2. Joar Franzén,
  3. Anders Näsman
  1. Department of oncology and pathology, Karolinska Institutet, Stockholm, Sweden
  1. Correspondence to Dr Anders Näsman, Department of Oncology and Pathology, Karolinska Institutet, Stockholm 177 76, Sweden; anders.nasman{at}


Keratoacanthomas (KA) and Spitz naevus (SN) are both lesions with unknown aetiology; therefore, the possibility of a viral involvement, more specifically the involvement of human polyomaviruses (HPyV), was investigated. In total, 22 cases of KA and 25 cases of SN were tested for the presence of HPyVs. DNA was extracted and amplified by multiplex PCR and thereafter tested with a multiplex bead-based assay for HPyVs (BKPyV, JCPyV, KIPyV, WUPyV, MCPyV, TSPyV, HPyV6, 7 and 9) and two primate viruses (SV40 and LPyV). HPyV DNA was found in 20 of the 47 lesions. There was no significant difference in HPyV DNA detection frequency between patients diagnosed with KA and patients diagnosed with SN, nor any over-representation of a specific HPyV type in any of the two patient categories. In conclusion, evidence for a specific aetiological role of any of the above tested HPyVs in either KA or SN was not disclosed.

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  • Handling editor Cheok Soon Lee

  • Contributors LH and AN planned the study. LH and JF ran the experiments. LH, JF and AN evaluated the data. LH, JF and AN. wrote and approved the manuscript.

  • Funding This work was financially supported by the Stockholm Council (SLL; clinical postdoctoral appointment), KI, Stiftelsen Sigurd och Elsa Goljes Minne and Magnus Bergvalls stiftelse.

  • Competing interests None.

  • Ethics approval 2012/83-31/2.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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