A novel molecular assay using hybridisation probes and melt curve analysis for CALR exon 9 mutation detection in myeloproliferative neoplasms

Thomas Keaney; Louise O'Connor; Janusz Krawczyk; Moutaz A Abdelrahman; Amjad H Hayat; Margaret Murray; Michael O'Dwyer; Melanie Percy; Stehpen Langabeer; Karl Haslam; Barry Glynn; Ciara Mullen; Evelyn Keady; Sinéad Lahiff; Terry J Smith

doi:10.1136/jclinpath-2016-204205

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A novel molecular assay using hybridisation probes and melt curve analysis for CALR exon 9 mutation detection in myeloproliferative neoplasms

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Thomas Keaney Research and Development Division, Advanced Molecular Systems, Galway, Ireland PubMed articles Google scholar articles
Louise O'Connor Research and Development Division, Advanced Molecular Systems, Galway, Ireland Molecular Diagnostic Research Group, School of Natural Sciences, National University of Ireland, Galway, Ireland PubMed articles Google scholar articles
Janusz Krawczyk Department of Haematology, National University of Ireland, Galway, Ireland Galway University Hospital, Galway, Ireland PubMed articles Google scholar articles
Moutaz A Abdelrahman Department of Haematology, National University of Ireland, Galway, Ireland Galway University Hospital, Galway, Ireland PubMed articles Google scholar articles
Amjad H Hayat Department of Haematology, National University of Ireland, Galway, Ireland Galway University Hospital, Galway, Ireland PubMed articles Google scholar articles
Margaret Murray Department of Haematology, National University of Ireland, Galway, Ireland Galway University Hospital, Galway, Ireland PubMed articles Google scholar articles
Michael O'Dwyer Department of Haematology, National University of Ireland, Galway, Ireland Galway University Hospital, Galway, Ireland PubMed articles Google scholar articles
Melanie Percy Department of Haematology, Belfast City Hospital, Belfast, UK PubMed articles Google scholar articles
Stehpen Langabeer Cancer Molecular Diagnostics, St. James's Hospital, Dublin, Ireland PubMed articles Google scholar articles
Karl Haslam Cancer Molecular Diagnostics, St. James's Hospital, Dublin, Ireland PubMed articles Google scholar articles
Barry Glynn Research and Development Division, Advanced Molecular Systems, Galway, Ireland PubMed articles Google scholar articles
Ciara Mullen Research and Development Division, Advanced Molecular Systems, Galway, Ireland PubMed articles Google scholar articles
Evelyn Keady Research and Development Division, Advanced Molecular Systems, Galway, Ireland PubMed articles Google scholar articles
Sinéad Lahiff Research and Development Division, Advanced Molecular Systems, Galway, Ireland PubMed articles Google scholar articles
Terry J Smith Research and Development Division, Advanced Molecular Systems, Galway, Ireland Molecular Diagnostic Research Group, School of Natural Sciences, National University of Ireland, Galway, Ireland PubMed articles Google scholar articles

Correspondence to Dr Louise O'Connor Molecular Diagnostics Research Group, Orbsen Building, National University of Ireland Galway, Galway, Ireland; louise.oconnor{at}nuigalway.ie

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Citation

Keaney T, O'Connor L, Krawczyk J, et al

A novel molecular assay using hybridisation probes and melt curve analysis for CALR exon 9 mutation detection in myeloproliferative neoplasms

Journal of Clinical Pathology 2017;70:662-668.

Publication history

Received October 27, 2016
Accepted December 15, 2016
First published January 31, 2017.

Online issue publication

July 19, 2017

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