Article Text

Download PDFPDF
FISH studies in DLBCL: correlations with cell of origin: the Irish experience
  1. Deirdre Mary Timlin1,
  2. Kevin O’Hare2,
  3. Jan Walker1,
  4. Giusseppa Castriciano1,
  5. Yvonne Connolly3,
  6. Cliona Grant3,
  7. Christopher Larry Bacon4,
  8. Elisabeth Vandenberghe4,
  9. Barbara Dunne1,
  10. Michael Jeffers1,2,
  11. Richard Flavin1
  1. 1 Histopathology Department, St James’s Hospital, Dublin, Ireland
  2. 2 Histopathology Department, Tallaght Hospital, Dublin, Ireland
  3. 3 Oncology Department, St James’s Hospital, Dublin, Ireland
  4. 4 Haematology Department, St James’s Hospital, Dublin, Ireland
  1. Correspondence to Dr Deirdre Mary Timlin, Histopathology Department, St James’s Hospital, Dublin 8, Ireland; dmtoomey{at}

Statistics from

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.

Diffuse large B cell lymphoma (DLBCL) is the most common non-Hodgkin’s lymphoma, and while the majority of patients will be successfully treated using immunochemotherapy, a significant number, up to 30%, will either fail primary therapy or quickly relapse. Gene expression profiling (GEP) and the identification of rearrangements (R) involving MYC, BCL2 and/or BCL6 can be used to stratify patients with DLBCL into prognostic subgroups. GEP identifies three molecular subgroups, germinal centre B (GCB), activated B (ABC) and type 3, which reflect stages of GCB cell development. Patients in the GCB subgroup have the best prognosis, while those of ABC subtype have inferior overall survival rates.1 This ‘cell of origin’ (COO) classification is now a mandatory requirement in the diagnostic work-up of DLBCL. GEP is expensive however and not widely available, in contrast to immunohistochemistry (IHC). As such, IHC algorithms based on B cell differentiation antigens were developed, which are shown to have variable degrees of concordance with GEP.2

High-grade B cell lymphoma with MYC, BCL2 and/or BCL6 R also characterise a distinct group of aggressive lymphomas, known as double or triple hit lymphomas (DH/TH), and can occur in 5%–10% of DLBCL cases.3 DH/TH are usually associated with the GCB …

View Full Text


  • Handling editor Mary Frances McMullin.

  • Contributors DMT and KO’H performed the audit. RF carried out the statistical tests. RF and DMT drafted the paper. RF, CLB, EV, MJ, BD, YC and CG were responsible for critical revision of the paper. JW and GC performed the tests.

  • Competing interests None declared.

  • Patient consent Not required.

  • Provenance and peer review Not commissioned; internally peer reviewed.