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Novel selective medium for the isolation of corynebacterium kroppenstedtii from heavily colonised clinical specimens
  1. Sally Cheuk Ying Wong1,2,
  2. Rosana W S Poon1,2,
  3. Chuen-Hing Foo1,
  4. Antonio H Y Ngan1,2,
  5. Herman Tse1,2,
  6. Vivian C M Lam1,2,
  7. Tiffany H Y Leung1,2,
  8. Chun-Pong Wong1,2,
  9. Vincent C C Cheng1,2,
  10. Jonathan H K Chen1,2,
  11. Kwok-Yung Yuen1,2
  1. 1 Department of Microbiology, Queen Mary Hospital, Hong Kong, China
  2. 2 Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China
  1. Correspondence to Professor Kwok-Yung Yuen, Carol Yu Centre for Infection, The University of Hong Kong, Queen Mary Hospital, Hong Kong, China; kyyuen{at}hku.hk

Abstract

Aims Granulomatous mastitis due to Corynebacterium kroppenstedtii is an increasingly recognised cause of an indolent and distressing mastitis in non-lactating females. This slow-growing lipophilic organism is not reliably isolated using routine culture methods. A novel selective culture medium (CKSM) is designed to optimise the isolation of this organism from clinical specimens.

Methods CKSM contains 10% galactose and Tween 80 (10%) to enhance the growth of C. kroppenstedtii, fosfomycin (100 µg/mL) to suppress the other bacteria, and differentiate C. kroppenstedtii from non-kroppenstedtii lipophilic corynebacteria by esculin hydrolysis. The medium was evaluated for its ability to support the growth of C. kroppenstedtii, selection and differentiation of C. kroppenstedtii from other bacteria in non-sterile clinical specimens.

Results C. kroppenstedtii grew as 1–2 mm colonies with black halo on CKSM within 72 hours of incubation, compared with barely visible pinpoint colonies on routine blood agars. During the four-month period of evaluation with 8896 respiratory specimens, 103 breast specimens, 1903 female genital tract specimens, 617 newborn surface swabs and 10 011 miscellaneous specimens, 186 C. kroppenstedtii were isolated, including 127 (1.4%) respiratory and 59 (0.5%) miscellaneous specimens, 184 of them were found only on CKSM. Besides the three (2.9%) positive breast specimens, 27 (1.4%) high vaginal and endocervical swabs, and 11 (1.8%) surface swabs of newborns were positive for C. kroppenstedtii.

Conclusions CKSM is a useful addition to routine agar media for the isolation of C. kroppenstedtii, and will be helpful for studying the epidemiology and transmission of this unusual Corynebacterium causing granulomatous mastitis.

  • corynebacteria
  • diagnostics
  • breast
  • infections

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Footnotes

  • Handling editor Tony Mazzulli.

  • Contributors SCW designed, performed analyses and wrote the manuscript. RWP designed, performed analyses and reviewed the manuscript. CHF, AHN, VCL, THL, CPW conducted experiments, collected data and reviewed the manuscript. HT, VCC performed analysis and reviewed the manuscript. KYY designed experiments, performed analysis and reviewed the manuscript.

  • Funding This study was supported by the Hong Kong University Foundation.

  • Competing interests None declared.

  • Patient consent Detail has been removed from this case description/these case descriptions to ensure anonymity.

  • Ethics approval Institutional Review Board of the University of Hong Kong/Hospital Authority Hong Kong West Cluster.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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