Aims The aim of the present study was to determine the concordant correlation in the expression of 88 target genes from triple-paired metastatic tissues in individual patients with metastatic renal carcinoma (mRCC) using a target gene sequencing (TGS) approach.
Methods Between 2002 and 2017, a total of 350 triple-paired metastatic tissue samples from 262 patients with mRCC obtained from either nephrectomy or metastasectomy were used for TGS of 88 candidate genes. After quality check, 243 tissue samples from 81 patients were finally applied to TGS. The concordance of triple-paired tissues was analysed with the 88 TGS panels using bioinformatics tools.
Results Among 81 patients, alterations were observed in 42 (51.9%) for any of the 88 mRCC panel genes; however, no pathogenic gene was detected in 38 (39.5%) . Concordance >95% for altered gene expression among the three tissues was reported in 12 (28.6%) patients, while concordance >95% within two tissues was reported in 30 (71.4%); concordance <50% was reported in the remaining eight patients. Considering several types of genetic alterations, including deletions, insertions, missense and nonsense mutations, and splice variants, genes most frequently detected with genetic alterations in the patients with mRCC were PTEN loss, followed by FLCN, BCR, SMARCA2, AKAP9, MLH1, MYH11, APC and TP53.
Conclusions The study provides reference information on the genetic alterations at various organ sites and the multi-heterogeneity of mRCC tissues. The concordance of pathogenic gene alterations within tissues was not high, and approximately half of the patients showed no pathogenic gene alterations at all.
- renal cell carcinoma
- gene sequencing
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Handling editor Runjan Chetty.
Contributors JC designed the study. SHK collected the data, performed the statistical analysis and wrote the manuscript. WSP performed all the pathological reviews. JC participated in the collection of the nephrectomy, metastasectomy specimen and helped to draft and subsequently review the manuscript.
Funding This study was supported by the Korean National Cancer Center (grant no.1710290-1).
Competing interests None declared.
Patient consent Not required.
Ethics approval National Cancer Center, Korea, NCC2015-0078.
Provenance and peer review Not commissioned; internally peer reviewed.
Data sharing statement The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.
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