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Solid and pseudopapillary neoplasms (SPNs) are rare tumours, their histogenesis is still a matter of debate. These tumours are reported to express CD-type proteins such as CD10, CD56 and CD99.1 We have recently observed another CD-type protein expression, that of CD138/syndecan-1, frequently used to point out a plasma cell differentiation.
The antibody used for the immunohistochemistry technique was the clone MI15 (IgG1 class) (Leica Biosystems, PA0088) reacting with human CD138 (immunogen: mixture of U266 and XG-1 human myeloma cell lines). The Leica Biosystems Bond III automated system has been used. Heat-induced epitope retrieval was performed with the citrate-based Bond Epitope Refine Solution-1 (30 min/mn). The Bond III Polymer Refine Detection system has been used as follows: …
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