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The Paris System for reporting urinary cytology in daily practice with emphasis on ancillary testing by multiprobe FISH
  1. Tatjana Vlajnic1,
  2. André Gut1,2,
  3. Spasenija Savic1,
  4. Lukas Bubendorf1
  1. 1 Institute of Pathology, University Hospital Basel, Basel, Switzerland
  2. 2 Institute of Pathology, Cantonal Hospital Winterthur, Winterthur, Switzerland
  1. Correspondence to Dr Tatjana Vlajnic, Institute of Pathology, University Hospital Basel, Basel 4031, Switzerland; tatjana.vlajnic{at}


Aims The Paris System (TPS) was introduced in the diagnostic routine with the goal to simplify and standardise diagnostic reporting of urinary cytology. The diagnostic categories of TPS are based on defined cytological criteria, with a focus on high-grade urothelial carcinoma (HGUC). While the categories ‘negative for HGUC (NHGUC)’ and ‘HGUC’ are straightforward, the categories ‘atypical urothelial cells (AUC)’ and ‘suspicious of HGUC (SHGUC)’ remain inconclusive. In this study, we evaluated the feasibility of TPS in daily practice with special emphasis on ancillary fluorescence in situ hybridisation (FISH) testing in the setting of TPS categories.

Methods In a 19-month period, TPS was prospectively applied in the routine diagnostic setting on 3900 urinary cytology cases comprising bladder and upper urinary tract washings and voided urine specimens. Additionally, we analysed the results of the FISH assay UroVysion prospectively performed on a cohort of 128 cases enriched for AUC and SHGUC categories.

Results The most frequently reported category was NHGUC (n=3496, 89.7%), followed by AUC (n=178, 4.6%), HGUC (n=155, 4%), SHGUC (n=61, 1.6%), low-grade urothelial neoplasia (n=6, 0.1%) and other malignancies (n=4, 0.1%). In the FISH cohort, 40/90 (44%) cases within the AUC category were FISH positive, consistent with urothelial neoplasia. In the SHGUC category, 16/21 (76%) cases were FISH positive.

Conclusions When prospectively applying TPS in urinary cytology, inconclusive atypia accounts only for a small subset of cases. FISH additionally improves the stratification between reactive and malignant cells in the indeterminate AUC and SHGUC categories.

  • Paris system
  • urinary cytology
  • urothelial carcinoma
  • atypical urothelial cells (AUC)
  • fluorescence in situ hybridisation

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  • Handling editor Runjan Chetty.

  • Contributors The study was designed by TV, SS and LB. The original draft of the manuscript was written by TV. The manuscript was written and critically revised by SS and LB. The collection of data was performed by all authors. The UroVysion FISH results were obtained and interpreted by TV, SS and LB. All authors read and approved the final manuscript.

  • Funding The authors have not declared a specific grant for this research from any funding agency in the public, commercial or not-for-profit sectors.

  • Competing interests None declared.

  • Patient consent for publication Not required.

  • Ethics approval The study has been exempt from Institutional Review Board (IRB) approval, since it served as a means of internal test validation and quality control.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Data availability statement All data relevant to the study are included in the article or uploaded as supplementary information.