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Overexpression of EI2BL promoted human non-small cell lung cancer progression by inducing cell EMT phenotype
  1. Hao-Ran Li1,
  2. Bai-Quan Qiu2,
  3. Jian Gao1,
  4. Chun Jin1,
  5. Jia-Hao Jiang1,
  6. Jian-Yong Ding1
  1. 1 Department of Thoracic Surgery, Zhongshan Hospital of Fudan University, Shanghai, China
  2. 2 Department of Cardiothoracic Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China
  1. Correspondence to Dr Jian-Yong Ding, Department of Thoracic Surgery, Zhongshan Hospital of Fudan University, Shanghai, China; ding.jianyong{at}


Aims To unveil the role of EI2BL in non-small cell lung cancer (NSCLC) and the relationship between expression of EI2BL and the prognosis of patients with NSCLC.

Methods Immunohistochemistry (IHC), western blot analysis, immunofluorescence and real-time quantitative PCR (RT-qPCR) were used to evaluate EI2BL protein and mRNA levels in NSCLC and corresponding peritumour tissues. Cell Counting Kit-8, transwell assay and wound healing assay were used to analyse the abilities of cell proliferation, invasion and migration. In addition, the analysis of epithelial-mesenchymal transition (EMT) markers was also assessed by western blot analysis, RT-qPCR and immunofluorescence. Tissue micro-array analysis of 200 NSCLC cases was used to assess the relationship between EI2BL expression and clinicopathological characteristics. Moreover, the prognostic role of EI2BL in 200 patients with NSCLC was evaluated by Cox regression models and Kaplan-Meier methods.

Results Elevated EI2BL expression was more common in NSCLC tissues than paired peritumour tissues in both mRNA and protein level. EI2BL promoted the proliferation, invasion and migration of NSCLC cells. In addition, aberrant EI2BL expression might modulate the expression of key molecules of EMT by ERK1/2 signal pathway. The expression of EI2BL was significantly associated with tumour stage, lymph node metastasis and tumour size. Moreover, higher expression of EI2BL in patients with NSCLC had a poor overall survival rate.

Conclusions Our study illustrated that elevated expression of EI2BL promoted NSCLC cell proliferation, migration and invasion and EI2BL overexpression may be a reliable biomarker of poor prognosis.

  • EI2BL
  • prognosis
  • EMT
  • biomarker

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  • Handling editor Cheok Soon Lee.

  • Contributors Conceptualisation: J-HJ and J-YD; formal analysis: CJ; funding acquisition: J-YD; methodology: H-RL; validation: H-RL, B-QQ and JG; writing—original draft: H-RL; writing—review and editing: H-RL and J-YD.

  • Funding This study was funded by Talent Funding Programme of Zhongshan Hospital of Fudan University (2017ZSGG02).

  • Competing interests None declared.

  • Patient consent for publication Not required.

  • Ethics approval All procedures performed in studies involving human participants were supported by Zhongshan Hospital Research Ethics Committee.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Data availability statement Data are available on reasonable request.